Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells

Makiko Yamashita; Yoshinori Katakura; Yoshihiro Aiba; Shin Ei Matsumoto; Kazuko Morihara; Kiichiro Teruya; Akira Ichikawa; Sanetaka Shirahata

doi:10.1007/s10616-007-9088-x

Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells

Makiko Yamashita, Yoshinori Katakura, Yoshihiro Aiba, Shin Ei Matsumoto, Kazuko Morihara, Kiichiro Teruya, Akira Ichikawa, Sanetaka Shirahata

Systems Biology

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Abstract

Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4⁺ Th cells and CD11c⁺ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.

Original language	English
Pages (from-to)	71-77
Number of pages	7
Journal	Cytotechnology
Volume	55
Issue number	2-3
DOIs	https://doi.org/10.1007/s10616-007-9088-x
Publication status	Published - Dec 2007

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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10.1007/s10616-007-9088-x

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title = "Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells",

abstract = "Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.",

author = "Makiko Yamashita and Yoshinori Katakura and Yoshihiro Aiba and Matsumoto, {Shin Ei} and Kazuko Morihara and Kiichiro Teruya and Akira Ichikawa and Sanetaka Shirahata",

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T1 - Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells

AU - Yamashita, Makiko

AU - Katakura, Yoshinori

AU - Aiba, Yoshihiro

AU - Matsumoto, Shin Ei

AU - Morihara, Kazuko

AU - Teruya, Kiichiro

AU - Ichikawa, Akira

AU - Shirahata, Sanetaka

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AB - Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.

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Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells

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