Investigation of the Separation Efficiency of Tube Radial Distribution Chromatography with Stationary Outer Phase Using the van Deemter Equation

Chihiro Matsushita, Kazuhiko Tsukagoshi, Katsumi Tsuchiya, Kenichi Yamashita, Masaharu Murata

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The outer phase in tube radial distribution chromatography (TRDC) operated as a stationary phase in chromatographic separation. Chromatographic peak information from model analytes 1-naphthol, 1-naphthalenesulfonic acid, 2,6-naphthalenedisulfonic acid, and 1,3,6-naphthalenetrisulfonic acid was examined using TRDC at various flow rates, using a fused-silica capillary tube (inner diameter, 75 μm; length, 120 cm; effective length, 100 cm) as the separation column and water/acetonitrile/ethyl acetate (3:8:4, v/v/v) as eluent. From the data, the relationships between average linear velocities and values of height equivalent to one theoretical plate of the analytes were investigated, and the separation efficiency by TRDC, comprising an inner phase (mobile phase) and outer phase (stationary phase) in an open-tubular capillary tube, was examined using the van Deemter equation.

Original languageEnglish
Pages (from-to)287-292
Number of pages6
JournalChromatographia
Volume83
Issue number2
DOIs
Publication statusPublished - Feb 1 2020

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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