Intracerebroventricular injection of pyrithiamine on short-term memory and habituation learning in mice

Satoshi Yamada, Shozo Tomonaga, D. Michael Denbow, Mitsuhiro Furuse

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1 Citation (Scopus)


Generally, experimental thiamine deficiency (TD) models involve feeding a thiamine deficient diet and giving intraperitoneal injections of the thiamine antagonist pyrithiamine (PT). While this method causes TD-like Wernicke-Korsakoff syndrome for 10-14 days, behavioral tests show that effects of these models include peripheral organic changes induced by systemic TD or chronic stress induced by housing conditions. In order to isolate the effects of TD due to alterations in the central nervous system, the effect of intracerebroventricular (i.c.v.) injection of PT on behavioral changes and monoamine and amino acid metabolism was investigated. In Experiment 1, short-term memory was not affected in the Y-maze test, but locomotor activity was decreased by PT. In the temporal lobe, monoamine metabolites were increased by PT. In Experiment 2, the effect of i.c.v. injection of PT on habituation learning behavior was investigated. The moving distance in the open field was significantly reduced in the control group, but not in the PT group. Monoamine metabolism was not changed by PT in the cerebral cortex, pons or hypothalamus, but norepinephrine and dopamine metabolites were significantly decreased in the hippocampus. In addition, PT caused a decreased in serotonin metabolites in the striatum and thalamus. These results suggest that alterations in central nervous system monoamine metabolism may be induced during the early stage of TD. These changes may be a pathological sign of thiamine deficiency.

Original languageEnglish
Pages (from-to)47-54
Number of pages8
JournalCurrent Topics in Nutraceutical Research
Issue number1-2
Publication statusPublished - Feb 2013

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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