TY - JOUR
T1 - Intracellular transport and processing of lysosomal cathepsin H
AU - Nishimura, Yukio
AU - Kato, Keitaro
N1 - Funding Information:
Research work was from supported the Ministry in part by of a Education, Grant- Japan and a Grant from the Uehara Memorial
PY - 1987/10/14
Y1 - 1987/10/14
N2 - Intracellular transport and processing of lysosomal cathepsin H was investigated in the subcellular fractions of rat liver by pulse-labeling experiments with [35S]methionine in vivo. A newly synthesized procathepsin H with a molecular weight of 41 kDa first appeared in the rough microsomal fraction at 10 min postinjection of label. This procathepsin H moved from the microsomal fraction to the Golgi subfractions at 30 min. Then a processed single-chain form with 28 kDa appeared in the lysosomal fraction at 60 min and persisted as the predominant form after 180 min. These results suggest that the propeptide-processing of procathepsin H indeed takes place in lysosomes in the course of intracellular transport from endoplasmic reticulum via Golgi complex to lysosomes.
AB - Intracellular transport and processing of lysosomal cathepsin H was investigated in the subcellular fractions of rat liver by pulse-labeling experiments with [35S]methionine in vivo. A newly synthesized procathepsin H with a molecular weight of 41 kDa first appeared in the rough microsomal fraction at 10 min postinjection of label. This procathepsin H moved from the microsomal fraction to the Golgi subfractions at 30 min. Then a processed single-chain form with 28 kDa appeared in the lysosomal fraction at 60 min and persisted as the predominant form after 180 min. These results suggest that the propeptide-processing of procathepsin H indeed takes place in lysosomes in the course of intracellular transport from endoplasmic reticulum via Golgi complex to lysosomes.
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U2 - 10.1016/0006-291X(87)91114-4
DO - 10.1016/0006-291X(87)91114-4
M3 - Article
C2 - 3675581
AN - SCOPUS:0023653430
SN - 0006-291X
VL - 148
SP - 329
EP - 334
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -