TY - JOUR
T1 - Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells
AU - Sonoda, Soichiro
AU - Yamaza, Haruyoshi
AU - Ma, Lan
AU - Tanaka, Yosuke
AU - Tomoda, Erika
AU - Aijima, Reona
AU - Nonaka, Kazuaki
AU - Kukita, Toshio
AU - Shi, Songtao
AU - Nishimura, Fusanori
AU - Yamaza, Takayoshi
N1 - Funding Information:
We are very thankful to Drs. Keitaro F. Masuda, Yusuke Makino, and Yoshihiro Hoshino (Kyushu University Hospital) for their technical support in this study. We also thank Ms. Megumi Ogawa (Department of Molecular Cell Biology and Oral Anatomy, Kyushu University) for her excellent assistance in this study. We are also thankful to Edanz Group Japan (Fukuoka, Japan) for their English language editing service to our manuscript. This work was supported by grants from the Japan Society for the Promotion of Science, including Grants-in-Aid for Scientific Research (B) (Grant Number 25293405 to TY) and Scientific Research (C) (Grant Number 25463187 to HY), Grant-in-Aid for Challenging Exploratory Research Project (Grant Number 24659815 to TY), and the Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers (Grant Number S2605 to FN).
PY - 2016/1/18
Y1 - 2016/1/18
N2 - Clinically, irreversible pulpitis is treated by the complete removal of pulp tissue followed by replacement with artificial materials. There is considered to be a high potential for autologous transplantation of human dental pulp stem cells (DPSCs) in endodontic treatment. The usefulness of DPSCs isolated from healthy teeth is limited. However, DPSCs isolated from diseased teeth with irreversible pulpitis (IP-DPSCs) are considered to be suitable for dentin/pulp regeneration. In this study, we examined the stem cell potency of IP-DPSCs. In comparison with healthy DPSCs, IP-DPSCs expressed lower colony-forming capacity, population-doubling rate, cell proliferation, multipotency, in vivo dentin regeneration, and immunosuppressive activity, suggesting that intact IP-DPSCs may be inadequate for dentin/pulp regeneration. Therefore, we attempted to improve the impaired in vivo dentin regeneration and in vitro immunosuppressive functions of IP-DPSCs to enable dentin/pulp regeneration. Interferon gamma (IFN-γ) treatment enhanced in vivo dentin regeneration and in vitro T cell suppression of IP-DPSCs, whereas treatment with tumor necrosis factor alpha did not. Therefore, these findings suggest that IFN-γ may be a feasible modulator to improve the functions of impaired IP-DPSCs, suggesting that autologous transplantation of IFN-γ-accelerated IP-DPSCs might be a promising new therapeutic strategy for dentin/pulp tissue engineering in future endodontic treatment.
AB - Clinically, irreversible pulpitis is treated by the complete removal of pulp tissue followed by replacement with artificial materials. There is considered to be a high potential for autologous transplantation of human dental pulp stem cells (DPSCs) in endodontic treatment. The usefulness of DPSCs isolated from healthy teeth is limited. However, DPSCs isolated from diseased teeth with irreversible pulpitis (IP-DPSCs) are considered to be suitable for dentin/pulp regeneration. In this study, we examined the stem cell potency of IP-DPSCs. In comparison with healthy DPSCs, IP-DPSCs expressed lower colony-forming capacity, population-doubling rate, cell proliferation, multipotency, in vivo dentin regeneration, and immunosuppressive activity, suggesting that intact IP-DPSCs may be inadequate for dentin/pulp regeneration. Therefore, we attempted to improve the impaired in vivo dentin regeneration and in vitro immunosuppressive functions of IP-DPSCs to enable dentin/pulp regeneration. Interferon gamma (IFN-γ) treatment enhanced in vivo dentin regeneration and in vitro T cell suppression of IP-DPSCs, whereas treatment with tumor necrosis factor alpha did not. Therefore, these findings suggest that IFN-γ may be a feasible modulator to improve the functions of impaired IP-DPSCs, suggesting that autologous transplantation of IFN-γ-accelerated IP-DPSCs might be a promising new therapeutic strategy for dentin/pulp tissue engineering in future endodontic treatment.
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U2 - 10.1038/srep19286
DO - 10.1038/srep19286
M3 - Article
C2 - 26775677
AN - SCOPUS:84954488912
SN - 2045-2322
VL - 6
JO - Scientific reports
JF - Scientific reports
M1 - 19286
ER -
