Interference Alignment (IA) emerged on the communication scene as a solution to the interference problem in all interference-limited networks, including heterogeneous cellular systems. However, the performance of IA is greatly related to the accuracy of the channel state information at transmitters (CSIT), namely the number of feedback bits. Accordingly, in order to improve the performance of IA, it would be useful to analyze the number of feedback bits with respect to the sum rate loss. Motivated by that, this paper studies a limited feedback-based IA scheme suitable for two tier macrocell-femtocell heterogeneous networks. First, an approximate analytical expression for the upper bound on the total sum rate loss due to limited feedback in the studied IA system, is derived. Then, a simulation based evaluation of the sum-rate loss due to the implementation of limited feedback IA in heterogeneous networks is obtained. Simulation results conﬁrmed the severe effect of quantization of CSI on the interference alignment performance.