Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow

Susumu Kudo, Makoto Hosobuchi, Shogo Kuretoko, Tateki Sumii, Tomoya Shimada, Marie Terada, Kazuo Tanishita

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


A phenomenon has been observed in which intracellular Ca 2+ concentration in endothelial cells increases upon application of shear stress (Ca 2+ response). It is therefore assumed that Ca 2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca 2+ response is also known to spread to surrounding cells (Ca 2+ wave). We investigated the effects on Ca 2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca 2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(l-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP 3) due to an intracellular IP 3 elevation. In addition, we investigated the Ca 2+ wave among BAECs under shear stress loading. Using Caged IP 3, local release of ATP from BAEC induced Ca 2+ wave. The Ca 2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca 2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.

Original languageEnglish
Pages (from-to)2431-2441
Number of pages11
JournalNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
Issue number784
Publication statusPublished - 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Condensed Matter Physics
  • Mechanical Engineering


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