Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors

Ryu Takeya, Koichiro Takeshige, Hideki Sumimoto

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)


We have cloned the cDNA encoding human PICK1 (protein interacting with C kinase 1), a PDZ domain-containing protein of 415 amino acids, and also identified the Drosophila homologue by search of the databank. Northern blot analysis shows a single mRNA of about 2.0 kb ubiquitously expressed in human tissues. Although PICK1 proteins harbor a region homologous to arfaptin1 and arfaptin2, two proteins that bind to the ARF (ADP-ribosylation factor), this region of PICK1 does not interact with ARFs in the yeast two-hybrid system. On the other hand, the PDZ domain of PICK1 is capable of interacting with constitutively active, GTP-bound forms of ARF1 and ARF3, but neither with those of ARF5/6 nor with the GDP-bound ARFs. The PICK1-ARF interaction is abrogated by introduction of mutations in the PDZ domain or by deletion of the extreme C-terminus of ARF1. Thus, PICK1 specifically interacts with ARF1/3 in the GTP-bound state, suggesting that PICK1 participates in ARF1/3-mediated cellular processes. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - Jan 7 2000

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors'. Together they form a unique fingerprint.

Cite this