TY - JOUR
T1 - Inhibition of porphobilinogen synthase activity by 4,5-dioxovalerate
AU - Shioi, Yuzo
AU - Doi, Michio
AU - Sasa, Tsutomu
N1 - Funding Information:
This research was supported in part by a Grants-in-Aid for Scientific Research from the Ministry of Science and Culture of Japan.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 1985/3
Y1 - 1985/3
N2 - The effect of 4,5-dioxovaleric acid on the activity of porphobilinogen (PBG) synthase (formerly 5-aminolevulinic acid dehydratase, EC 4.2.1.24) of the porphyrin synthetic pathway was studied with the enzyme purified from Chlorella regularis. 4,5-Dioxovaleric acid, a metabolite of 5-aminolevulinic acid, competitively inhibited PBG synthase with a Ki value of 1.4 mm. The concentration for the half inhibition of 4,5-dioxovaleric acid (7 mm) was slightly lower than that for the known competitive inhibitor, levulinic acid (12 mm).
AB - The effect of 4,5-dioxovaleric acid on the activity of porphobilinogen (PBG) synthase (formerly 5-aminolevulinic acid dehydratase, EC 4.2.1.24) of the porphyrin synthetic pathway was studied with the enzyme purified from Chlorella regularis. 4,5-Dioxovaleric acid, a metabolite of 5-aminolevulinic acid, competitively inhibited PBG synthase with a Ki value of 1.4 mm. The concentration for the half inhibition of 4,5-dioxovaleric acid (7 mm) was slightly lower than that for the known competitive inhibitor, levulinic acid (12 mm).
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M3 - Article
AN - SCOPUS:77958430928
SN - 0032-0781
VL - 26
SP - 379
EP - 382
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 2
ER -