TY - JOUR
T1 - Inhibition of atrial natriuretic peptide secretion by forskolin in noncontracting cultured atrial myocytes
AU - Iida, Hiroshi
AU - Page, Ernest
N1 - Funding Information:
This work was supported by USPHS grants HL 20592 and HL 10503.
PY - 1988/11/30
Y1 - 1988/11/30
N2 - Secretory rates for immunoreactive atrial natriuretic peptide (ANP) by 7-8 day-old primary cultures of atrial myocytes from adult rats (with myocyte contraction inhibited by tetrodotoxin (TTX)) were (a) constant for at least two hours, and (b) significantly slowed by forskolin (1, 5, and 25 μM), dibutyryl cyclic adenosine monophosphate (1 mM), or isobutylmethylxanthine (100 μM). The substantial rates of ANP secretion which persisted in cells rendered noncontracting either by inhibiting Ca2+ influx via reduction of external [Ca2+] to <10-7 M or by inhibiting sarcoplasmic reticulum Ca2+ release with 100 μM ryanodine were significantly slowed by 25 μM forskolin, but forskolin sensitivity was lost by cells exposed simultaneously to external Ca2+ concentration of <10-7 M and 100 μM ryanodine. Quiescent myocytes whose ANP secretory rate was depressed by forskolin remained responsive to secretory stimulation by phorbol ester.
AB - Secretory rates for immunoreactive atrial natriuretic peptide (ANP) by 7-8 day-old primary cultures of atrial myocytes from adult rats (with myocyte contraction inhibited by tetrodotoxin (TTX)) were (a) constant for at least two hours, and (b) significantly slowed by forskolin (1, 5, and 25 μM), dibutyryl cyclic adenosine monophosphate (1 mM), or isobutylmethylxanthine (100 μM). The substantial rates of ANP secretion which persisted in cells rendered noncontracting either by inhibiting Ca2+ influx via reduction of external [Ca2+] to <10-7 M or by inhibiting sarcoplasmic reticulum Ca2+ release with 100 μM ryanodine were significantly slowed by 25 μM forskolin, but forskolin sensitivity was lost by cells exposed simultaneously to external Ca2+ concentration of <10-7 M and 100 μM ryanodine. Quiescent myocytes whose ANP secretory rate was depressed by forskolin remained responsive to secretory stimulation by phorbol ester.
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U2 - 10.1016/S0006-291X(88)80051-2
DO - 10.1016/S0006-291X(88)80051-2
M3 - Article
C2 - 2461711
AN - SCOPUS:0024226661
SN - 0006-291X
VL - 157
SP - 330
EP - 336
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -