TY - JOUR
T1 - Influences of cholinergic neurotoxin ethylcholine aziridinium ion on circadian rhythms in rats
AU - Endo, Yutaka
AU - Shinohara, Kazuyuki
AU - Fueta, Yukiko
AU - Irie, Masahiro
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science and Culture, Japan (No. 06770786) to Y. Endo.
PY - 2001
Y1 - 2001
N2 - To investigate whether damages of cholinergic neurons in the brain produce aging-like changes in circadian rhythms, we examined the influences of intracerebroventricular injection of cholinergic neurotoxin ethylcholine aziridinium ion (AF64A, 5 nmol/5 μl) on circadian rhythms in rats, by measuring locomotor activity and body temperature with the automatic behavioral measurement system combined with the telemetry. Daily rhythms in locomotor activity and body temperature were observed in AF64A-treated rats under a 12:12 h light:dark (LD) cycle, however, in AF64A-treated rats, the amplitude of activity and temperature rhythms was significantly decreased, the phase of the both rhythms was advanced and the amount of activity was decreased, compared with control rats. Locomotor activity and body temperature also showed a circadian rhythm in AF64A-treated rats under the constant dark condition with the period similar to that in the control rats. The present findings are in accordance with the observation in aged animals in which cholinergic hypofunction are often observed, suggesting that hypofunctions of the cholinergic systems in the brain might be involved in aging-like changes in the circadian rhythms.
AB - To investigate whether damages of cholinergic neurons in the brain produce aging-like changes in circadian rhythms, we examined the influences of intracerebroventricular injection of cholinergic neurotoxin ethylcholine aziridinium ion (AF64A, 5 nmol/5 μl) on circadian rhythms in rats, by measuring locomotor activity and body temperature with the automatic behavioral measurement system combined with the telemetry. Daily rhythms in locomotor activity and body temperature were observed in AF64A-treated rats under a 12:12 h light:dark (LD) cycle, however, in AF64A-treated rats, the amplitude of activity and temperature rhythms was significantly decreased, the phase of the both rhythms was advanced and the amount of activity was decreased, compared with control rats. Locomotor activity and body temperature also showed a circadian rhythm in AF64A-treated rats under the constant dark condition with the period similar to that in the control rats. The present findings are in accordance with the observation in aged animals in which cholinergic hypofunction are often observed, suggesting that hypofunctions of the cholinergic systems in the brain might be involved in aging-like changes in the circadian rhythms.
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U2 - 10.1016/S0168-0102(01)00295-4
DO - 10.1016/S0168-0102(01)00295-4
M3 - Article
C2 - 11755225
AN - SCOPUS:0035654686
SN - 0168-0102
VL - 41
SP - 385
EP - 390
JO - Neuroscience Research
JF - Neuroscience Research
IS - 4
ER -