Influence of plasma-activated compounds on melanogenesis and tyrosinase activity

Anser Ali, Zaman Ashraf, Naresh Kumar, Muhammad Rafiq, Farukh Jabeen, Ji Hoon Park, Ki Hong Choi, Seunghyun Lee, Sung Yum Seo, Eun Ha Choi, Pankaj Attri

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


Many organic chemists around the world synthesize medicinal compounds or extract multiple compounds from plants in order to increase the activity and quality of medicines. In this work, we synthesized new eugenol derivatives (ED) and then treated them with an N2 feeding gas atmospheric pressure plasma jet (APPJ) to increase their utility. We studied the tyrosinase-inhibition activity (activity test) and structural changes (circular dichroism) of tyrosinase with ED and plasma activated eugenol derivatives (PAED) in a cell-free environment. Later, we used docking studies to determine the possible interaction sites of ED and PAED compounds with tyrosinase enzyme. Moreover, we studied the possible effect of ED and PAED on melanin synthesis and its mechanism in melanoma (B16F10) cells. Additionally, we investigated the structural changes that occurred in activated ED after plasma treatment using nuclear magnetic resonance (NMR). Hence, this study provides a new perspective on PAED for the field of plasma medicine.

Original languageEnglish
Article number21779
JournalScientific reports
Publication statusPublished - Mar 2 2016

All Science Journal Classification (ASJC) codes

  • General


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