TY - JOUR
T1 - Induction of pluripotency in mammalian fibroblasts by cell fusion with mouse embryonic stem cells
AU - Imai, Hiroyuki
AU - Kusakabe, Ken Takeshi
AU - Kiso, Yasuo
AU - Hattori, Shosaku
AU - Kai, Chieko
AU - Ono, Etsuro
AU - Kano, Kiyoshi
N1 - Funding Information:
The authors thank Dr. Nobuhide Kido (Kanazawa Zoological Gardens) for the gift of an aborted placenta of black rhinoceros, Taiki Matsuo and Prof. Naomi Wada (Veterinary System Physiology, Yamaguchi University) for autopsied tissues, and Prof. Toshio Kitamura (Institute of Medical Science, the University of Tokyo) for pMCs-puro. This work was supported by JSPS KAKENHI; Grant Numbers JP25660254, JP15K14880, JP17J07902, grants-in-aid from the Foundation for Growth Science and grant for Joint Research Project of Institute of Medical Science, the University of Tokyo; Number 3015.
Funding Information:
The authors thank Dr. Nobuhide Kido (Kanazawa Zoological Gardens) for the gift of an aborted placenta of black rhinoceros, Taiki Matsuo and Prof. Naomi Wada (Veterinary System Physiology, Yamaguchi University) for autopsied tissues, and Prof. Toshio Kitamura (Institute of Medical Science, the University of Tokyo) for pMCs-puro. This work was supported by JSPS KAKENHI ; Grant Numbers JP25660254 , JP15K14880 , JP17J07902 , grants-in-aid from the Foundation for Growth Science and grant for Joint Research Project of Institute of Medical Science, the University of Tokyo ; Number 3015 .
Publisher Copyright:
© 2019
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Background: Cell fusion is a phenomenon that is observed in various tissues in vivo, resulting in acquisition of physiological functions such as liver regeneration. Fused cells such as hybridomas have also been produced artificially in vitro. Furthermore, it has been reported that cellular reprogramming can be induced by cell fusion with stem cells. Methods: Fused cells between mammalian fibroblasts and mouse embryonic stem cells were produced by electrofusion methods. The phenotypes of each cell lines were analyzed after purifying the fused cells. Results: Colonies which are morphologically similar to mouse embryonic stem cells were observed in fused cells of rabbit, bovine, and zebra fibroblasts. RT-PCR analysis revealed that specific pluripotent marker genes that were never expressed in each mammalian fibroblast were strongly induced in the fused cells, which indicated that fusion with mouse embryonic stem cells can trigger reprogramming and acquisition of pluripotency in various mammalian somatic cells. Conclusions: Our results can help elucidate the mechanism of pluripotency maintenance and the establishment of highly reprogrammed pluripotent stem cells in various mammalian species.
AB - Background: Cell fusion is a phenomenon that is observed in various tissues in vivo, resulting in acquisition of physiological functions such as liver regeneration. Fused cells such as hybridomas have also been produced artificially in vitro. Furthermore, it has been reported that cellular reprogramming can be induced by cell fusion with stem cells. Methods: Fused cells between mammalian fibroblasts and mouse embryonic stem cells were produced by electrofusion methods. The phenotypes of each cell lines were analyzed after purifying the fused cells. Results: Colonies which are morphologically similar to mouse embryonic stem cells were observed in fused cells of rabbit, bovine, and zebra fibroblasts. RT-PCR analysis revealed that specific pluripotent marker genes that were never expressed in each mammalian fibroblast were strongly induced in the fused cells, which indicated that fusion with mouse embryonic stem cells can trigger reprogramming and acquisition of pluripotency in various mammalian somatic cells. Conclusions: Our results can help elucidate the mechanism of pluripotency maintenance and the establishment of highly reprogrammed pluripotent stem cells in various mammalian species.
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U2 - 10.1016/j.bbrc.2019.10.026
DO - 10.1016/j.bbrc.2019.10.026
M3 - Article
C2 - 31635800
AN - SCOPUS:85073729015
SN - 0006-291X
VL - 521
SP - 24
EP - 30
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -