Induction of apoptosis by tocotrienol in rat hepatoma dRLh-84 cells

Midori Sakai, Masaaki Okabe, Masao Yamasaki, Hirofumi Tachibana, Koji Yamada

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42 Citations (Scopus)


Our aim was to evaluate the antitumor activities of tocopherol (Toc) and tocotrienol (T3) derivatives. At first, we examined the effect of these vitamin E homologues on the proliferation of rat normal hepatocyte RLN-10 and hepatoma dRLh-84 cells and found that especially T3 inhibited cell proliferation in dRLh-84 cells. Then, we examined the effect of vitamin E homologues on apoptosis induction and found that T3 induced DNA fragmentation and stimulated a rise of caspase-3 activity. In addition, T3 stimulated a rise in caspase-8 activity, while a caspase-8 inhibitor suppressed apoptosis induction by T3. We also examined the incorporation of vitamin E homologues into dRLh-84 cells. T3 was incorporated more quickly, compared to Toc. These results indicated that T3 induces apoptosis in dRLh-84 cells and that caspase-8 is involved in this apoptosis induction. The difference in terms of apoptosis induction by vitamin E homologues seems to be related to their different rates of cellular incorporation.

Original languageEnglish
Pages (from-to)1683-1688
Number of pages6
JournalAnticancer research
Issue number3 A
Publication statusPublished - May 2004

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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