TY - JOUR
T1 - Increased biofilm formation ability and accelerated transport of Staphylococcus aureus along a catheter during reciprocal movements
AU - Haraga, Isao
AU - Abe, Shintaro
AU - Jimi, Shiro
AU - Kiyomi, Fumiaki
AU - Yamaura, Ken
N1 - Funding Information:
The authors would like to thank Dr. K. Higa (Professor Emeritus at Department of Anesthesiology, Fukuoka University Faculty of Medicine) for comments on the manuscript; Dr. H. HANAKI (Kitasato University, Laboratory of Infection Control and Research Center for Infections and Antimicrobials, Japan) for comments on the manuscript and the provision of strains ATCC 25923, ATCC 29213, ATCC 700698, and ATCC 700699; Dr. T. TAKATA (Fukuoka University Hospital, Department of Infection Control, Fukuoka, Japan) and H. Ohjimi (Department of Plastic, Reconstructive and Aesthetic Surgery, Fukuoka University, Fukuoka, Japan) for the provision of strain Oj-one; Medical English Service ( www.med-english.com ) for the English language comments; Enago ( www.enago.jp ) for the English language review. This study was funded by the Department of Anesthesiology, Fukuoka University Faculty of Medicine and the Department of Intellectual Property, Fukuoka University , Fukuoka, Japan.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Staphylococcus spp. is a major cause of device-related infections. However, the mechanisms of deep-tissue infection by staphylococci from the skin surface remain unclear. We performed in vitro experiments to determine how staphylococci are transferred from the surface to the deeper layers of agar along the catheter for different strains of Staphylococcus aureus with respect to bacterial concentrations, catheter movements, and biofilm formation. We found that when 5-mm reciprocal movements of the catheter were repeated every 8 h, all catheter samples of S. aureus penetrated the typical distance of 50 mm from the skin to the epidural space. The number of reciprocal catheter movements and the depth of bacterial growth were correlated. A greater regression coefficient for different strains implied faster bacterial growth. Enhanced biofilm formation by different strains implied larger regression coefficients. Increased biofilm formation ability may accelerate S. aureus transport along a catheter due to physical movements by patients.
AB - Staphylococcus spp. is a major cause of device-related infections. However, the mechanisms of deep-tissue infection by staphylococci from the skin surface remain unclear. We performed in vitro experiments to determine how staphylococci are transferred from the surface to the deeper layers of agar along the catheter for different strains of Staphylococcus aureus with respect to bacterial concentrations, catheter movements, and biofilm formation. We found that when 5-mm reciprocal movements of the catheter were repeated every 8 h, all catheter samples of S. aureus penetrated the typical distance of 50 mm from the skin to the epidural space. The number of reciprocal catheter movements and the depth of bacterial growth were correlated. A greater regression coefficient for different strains implied faster bacterial growth. Enhanced biofilm formation by different strains implied larger regression coefficients. Increased biofilm formation ability may accelerate S. aureus transport along a catheter due to physical movements by patients.
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U2 - 10.1016/j.mimet.2016.11.003
DO - 10.1016/j.mimet.2016.11.003
M3 - Article
C2 - 27836632
AN - SCOPUS:84995877202
SN - 0167-7012
VL - 132
SP - 63
EP - 68
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
ER -