Increase of immunoglobulin productivity of human-human hybridoma HB4C5 cells by histone

Takeaki Okamoto, Takuya Sugahara, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


A histone mixture (H1, H2A, H2B, H3, and H4) derived from calf thymus stimulated IgM production by human-human hybridoma HB4C5 cells. On the contrary, the histone mixture did not increase IgM production by the human Burkitt's lymphoma cell line NAT-30, IgG production by the human B lymphoblastoid cell line HMy-2, and IgE production by the human myeloma cell line U266. The immunoglobulin production-stimulating activity of the histone mixture was inactivated by trypsin or chymotrypsin digestion. In addition, confocal laser microscopic analysis had shown that HB4C5 cells incorporated a lot of histone but other cell lines did not incorporate it as much. These facts strongly suggest that histone acts as an immunoglobulin production-stimulating factor (IPSF) after internalization into the human B cell lines and the native structure of histone is required for the IPSF activity.

Original languageEnglish
Pages (from-to)1241-1245
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Issue number6
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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