Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea

Kazunori Tanaka; Tasuku Shimizu; Muhammad Zakria; Joyce Njoloma; Yuichi Saeki; Masao Sakai; Takeo Yamakawa; Kiwamu Minamisawa; Shoichiro Akao

doi:10.1264/jsme2.21.122

Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea

Kazunori Tanaka, Tasuku Shimizu, Muhammad Zakria, Joyce Njoloma, Yuichi Saeki, Masao Sakai, Takeo Yamakawa, Kiwamu Minamisawa, Shoichiro Akao

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Three endophytic bacterial strains (Burkholderia sp. No. 25, Enterobacter sp. No. 35 and Klebsiella sp. No. 38) isolated from sweet potato and sugarcane were examined for their ability to incorporate the gene encoding green fluorescent protein (GFP) via conjugation and electroporation. Enterobacter sp. No. 35 and Klebsiella sp. No. 38 were successfully tagged with the gfp gene by conjugation using pTn5kmgfpmutl. Fluorescence microscopic observation of Brassica oleracea inoculated with gfp-tagged endophytes revealed that Enterobacter sp. No. 35 and Klebsiella sp. No. 38 colonized the junctions between the lateral roots and the main roots.

Original language	English
Pages (from-to)	122-128
Number of pages	7
Journal	Microbes and environments
Volume	21
Issue number	2
DOIs	https://doi.org/10.1264/jsme2.21.122
Publication status	Published - 2006

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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Tanaka, K., Shimizu, T., Zakria, M., Njoloma, J., Saeki, Y., Sakai, M., Yamakawa, T., Minamisawa, K., & Akao, S. (2006). Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea. Microbes and environments, 21(2), 122-128. https://doi.org/10.1264/jsme2.21.122

Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea. / Tanaka, Kazunori; Shimizu, Tasuku; Zakria, Muhammad et al.
In: Microbes and environments, Vol. 21, No. 2, 2006, p. 122-128.

Research output: Contribution to journal › Article › peer-review

Tanaka, K, Shimizu, T, Zakria, M, Njoloma, J, Saeki, Y, Sakai, M, Yamakawa, T, Minamisawa, K & Akao, S 2006, 'Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea', Microbes and environments, vol. 21, no. 2, pp. 122-128. https://doi.org/10.1264/jsme2.21.122

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abstract = "Three endophytic bacterial strains (Burkholderia sp. No. 25, Enterobacter sp. No. 35 and Klebsiella sp. No. 38) isolated from sweet potato and sugarcane were examined for their ability to incorporate the gene encoding green fluorescent protein (GFP) via conjugation and electroporation. Enterobacter sp. No. 35 and Klebsiella sp. No. 38 were successfully tagged with the gfp gene by conjugation using pTn5kmgfpmutl. Fluorescence microscopic observation of Brassica oleracea inoculated with gfp-tagged endophytes revealed that Enterobacter sp. No. 35 and Klebsiella sp. No. 38 colonized the junctions between the lateral roots and the main roots.",

author = "Kazunori Tanaka and Tasuku Shimizu and Muhammad Zakria and Joyce Njoloma and Yuichi Saeki and Masao Sakai and Takeo Yamakawa and Kiwamu Minamisawa and Shoichiro Akao",

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AU - Tanaka, Kazunori

AU - Shimizu, Tasuku

AU - Zakria, Muhammad

AU - Njoloma, Joyce

AU - Saeki, Yuichi

AU - Sakai, Masao

AU - Yamakawa, Takeo

AU - Minamisawa, Kiwamu

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AB - Three endophytic bacterial strains (Burkholderia sp. No. 25, Enterobacter sp. No. 35 and Klebsiella sp. No. 38) isolated from sweet potato and sugarcane were examined for their ability to incorporate the gene encoding green fluorescent protein (GFP) via conjugation and electroporation. Enterobacter sp. No. 35 and Klebsiella sp. No. 38 were successfully tagged with the gfp gene by conjugation using pTn5kmgfpmutl. Fluorescence microscopic observation of Brassica oleracea inoculated with gfp-tagged endophytes revealed that Enterobacter sp. No. 35 and Klebsiella sp. No. 38 colonized the junctions between the lateral roots and the main roots.

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Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea

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