Inactivation of Enzymes in an Aqueous Solution by Micro-Bubbles of Supercritical Carbon Dioxide

Hiroya Ishikawa; Mitsuya Shimoda; Yutaka Osajima; Tamotsu Kawano

doi:10.1271/bbb.59.628

Inactivation of Enzymes in an Aqueous Solution by Micro-Bubbles of Supercritical Carbon Dioxide

Hiroya Ishikawa, Mitsuya Shimoda, Yutaka Osajima, Tamotsu Kawano

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

Enzyme solutions of glucoamylase, acid protease, alkaline protease and lipase were treated with micro-bubbles of supercritical carbon dioxide (SC CO₂) fed from a cylindrical filter nozzle. The micro-bubbles of SC CO₂ could increase the CO₂ concentration in the sample solution from 0.4 to 0.92 mol/1 at 25 MPa and 35°C, and hence could improve the efficiency of inactivation by about 3 times compared to treating without the filter nozzle. Alkaline protease and lipase in the solution could be completely inactivated by the treatment at 35°C and 15 MPa for 30 min. With the inactivation of glucoamylase and acid protease, their residual activity-CO₂ density profiles consisted of two straight lines with intersections at density values of 0.82 and 0.60 g/cm³. respectively. These enzymes above the CO₂ density of their intersections.

Original language	English
Pages (from-to)	628-631
Number of pages	4
Journal	Bioscience, biotechnology, and biochemistry
Volume	59
Issue number	4
DOIs	https://doi.org/10.1271/bbb.59.628
Publication status	Published - 1995

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.59.628

Cite this

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abstract = "Enzyme solutions of glucoamylase, acid protease, alkaline protease and lipase were treated with micro-bubbles of supercritical carbon dioxide (SC CO2) fed from a cylindrical filter nozzle. The micro-bubbles of SC CO2 could increase the CO2 concentration in the sample solution from 0.4 to 0.92 mol/1 at 25 MPa and 35°C, and hence could improve the efficiency of inactivation by about 3 times compared to treating without the filter nozzle. Alkaline protease and lipase in the solution could be completely inactivated by the treatment at 35°C and 15 MPa for 30 min. With the inactivation of glucoamylase and acid protease, their residual activity-CO2 density profiles consisted of two straight lines with intersections at density values of 0.82 and 0.60 g/cm3. respectively. These enzymes above the CO2 density of their intersections.",

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T1 - Inactivation of Enzymes in an Aqueous Solution by Micro-Bubbles of Supercritical Carbon Dioxide

AU - Ishikawa, Hiroya

AU - Shimoda, Mitsuya

AU - Osajima, Yutaka

AU - Kawano, Tamotsu

PY - 1995

Y1 - 1995

N2 - Enzyme solutions of glucoamylase, acid protease, alkaline protease and lipase were treated with micro-bubbles of supercritical carbon dioxide (SC CO2) fed from a cylindrical filter nozzle. The micro-bubbles of SC CO2 could increase the CO2 concentration in the sample solution from 0.4 to 0.92 mol/1 at 25 MPa and 35°C, and hence could improve the efficiency of inactivation by about 3 times compared to treating without the filter nozzle. Alkaline protease and lipase in the solution could be completely inactivated by the treatment at 35°C and 15 MPa for 30 min. With the inactivation of glucoamylase and acid protease, their residual activity-CO2 density profiles consisted of two straight lines with intersections at density values of 0.82 and 0.60 g/cm3. respectively. These enzymes above the CO2 density of their intersections.

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Inactivation of Enzymes in an Aqueous Solution by Micro-Bubbles of Supercritical Carbon Dioxide

Abstract

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