Inactivation of enzymes in an aqueous solution by micro-bubbles of supercritical carbon dioxide

Hiroya Ishikawa, Mitsuya Shimoda, Tamotsu Kawano, Yutaka Osajima

Research output: Contribution to journalArticlepeer-review


Enzyme solutions of glucoamylase, acid protease, alkaline protease and lipase were treated with micro-bubbles of supercritical carbon dioxide (SC CO2) fed from a cylindrical filter nozzle. The micro-bubbles of SC CO2 could increase the CO2 concentration in the sample solution from 0.4 to 0.92 mol/l at 25 MPa and 35°C, and hence could improve the efficiency of inactivation by about 3 times compared to treating without the filter nozzle. Alkaline protease and lipase in the solution could be completely inactivated by the treatment at 35°C and 15 MPa for 30 min. With the inactivation of glucoamylase and acid protease, their residual activity-CO2 density profiles consisted of two straight lines with intersections at density values of 0.82 and 0.60 g/cm3, respectively. These enzymes showed an abrupt decrease in activation in regions above the CO2 density of their intersections.

Original languageEnglish
Pages (from-to)628-631
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number4
Publication statusPublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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