Inactivation of bacillus spores by the supercritical carbon dioxide micro-bubble method

Hiroya Ishikawa; Mitsuya Shimoda; Kei Tamaya; Akiyoshi Yonekura; Tamotsu Kawano; Yutaka Osajima

doi:10.1271/bbb.61.1022

Inactivation of bacillus spores by the supercritical carbon dioxide micro-bubble method

Hiroya Ishikawa, Mitsuya Shimoda, Kei Tamaya, Akiyoshi Yonekura, Tamotsu Kawano, Yutaka Osajima

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

Bacillus spores were effectively inactivated by the supercritical (SC) CO₂ micro-bubble method. The micro-bubble SC CO₂ treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40°C and 30 MPa for 30min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO₂ treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45°C, 50°C, and 55°C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO₂ treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100°C for 60 min. In addition, the SC CO₂ treatment (30 MPa, 60°C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.

Original language	English
Pages (from-to)	1022-1023
Number of pages	2
Journal	Bioscience, Biotechnology and Biochemistry
Volume	61
Issue number	6
DOIs	https://doi.org/10.1271/bbb.61.1022
Publication status	Published - Jan 1997

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.61.1022

Cite this

@article{c42d8fef84ba42539cef571a65b9e506,

title = "Inactivation of bacillus spores by the supercritical carbon dioxide micro-bubble method",

abstract = "Bacillus spores were effectively inactivated by the supercritical (SC) CO2 micro-bubble method. The micro-bubble SC CO2 treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40°C and 30 MPa for 30min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO2 treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45°C, 50°C, and 55°C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO2 treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100°C for 60 min. In addition, the SC CO2 treatment (30 MPa, 60°C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.",

author = "Hiroya Ishikawa and Mitsuya Shimoda and Kei Tamaya and Akiyoshi Yonekura and Tamotsu Kawano and Yutaka Osajima",

year = "1997",

month = jan,

doi = "10.1271/bbb.61.1022",

language = "English",

volume = "61",

pages = "1022--1023",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "6",

}

TY - JOUR

T1 - Inactivation of bacillus spores by the supercritical carbon dioxide micro-bubble method

AU - Ishikawa, Hiroya

AU - Shimoda, Mitsuya

AU - Tamaya, Kei

AU - Yonekura, Akiyoshi

AU - Kawano, Tamotsu

AU - Osajima, Yutaka

PY - 1997/1

Y1 - 1997/1

N2 - Bacillus spores were effectively inactivated by the supercritical (SC) CO2 micro-bubble method. The micro-bubble SC CO2 treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40°C and 30 MPa for 30min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO2 treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45°C, 50°C, and 55°C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO2 treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100°C for 60 min. In addition, the SC CO2 treatment (30 MPa, 60°C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.

AB - Bacillus spores were effectively inactivated by the supercritical (SC) CO2 micro-bubble method. The micro-bubble SC CO2 treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40°C and 30 MPa for 30min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO2 treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45°C, 50°C, and 55°C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO2 treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100°C for 60 min. In addition, the SC CO2 treatment (30 MPa, 60°C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.

UR - http://www.scopus.com/inward/record.url?scp=0031171095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031171095&partnerID=8YFLogxK

U2 - 10.1271/bbb.61.1022

DO - 10.1271/bbb.61.1022

M3 - Article

C2 - 9214765

AN - SCOPUS:0031171095

SN - 0916-8451

VL - 61

SP - 1022

EP - 1023

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 6

ER -

Inactivation of bacillus spores by the supercritical carbon dioxide micro-bubble method

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this