Inactivation of bacillus spores by the supercritical carbon dioxide micro-bubble method

Hiroya Ishikawa, Mitsuya Shimoda, Kei Tamaya, Akiyoshi Yonekura, Tamotsu Kawano, Yutaka Osajima

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76 Citations (Scopus)


Bacillus spores were effectively inactivated by the supercritical (SC) CO2 micro-bubble method. The micro-bubble SC CO2 treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40°C and 30 MPa for 30min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO2 treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45°C, 50°C, and 55°C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO2 treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100°C for 60 min. In addition, the SC CO2 treatment (30 MPa, 60°C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.

Original languageEnglish
Pages (from-to)1022-1023
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Issue number6
Publication statusPublished - Jan 1997

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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