TY - JOUR
T1 - In vitroandin vivoblood compatibility of concentrated polymer brushes
AU - Yoshikawa, Chiaki
AU - Hattori, Shinya
AU - Huang, Chih Feng
AU - Kobayashi, Hisatoshi
AU - Tanaka, Masaru
N1 - Funding Information:
This work was performed in part on the NIMS Molecular and Material Synthesis Platform. This work was supported by JSPS KAKENHI Grant Numbers JP18K05249 (C. Yoshikawa) and JP19H05720 (M. Tanaka), and performed under the Cooperative Research Program of ‘‘Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials’’.
Funding Information:
This work was performed in part on the NIMS Molecular and Material Synthesis Platform. This work was supported by JSPS KAKENHI Grant Numbers JP18K05249 (C. Yoshikawa) and JP19H05720 (M. Tanaka), and performed under the Cooperative Research Program of ?Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials?.
Publisher Copyright:
© The Royal Society of Chemistry 2021.
PY - 2021/8/7
Y1 - 2021/8/7
N2 - Concentrated polymer brushes (CPBs) and semi-dilute polymer brushes (SDPBs) of poly(2-hydroxyethyl methacrylate), poly(2-hydroxyethyl acrylate), poly[poly(ethylene glycol)methyl ether methacrylate] (PPEGMA) and poly(2-methoxyetyl acrylate) were prepared on silica particles and silicon wafers by surface-initiated atom transfer radical polymerization (SI-ATRP). In order to evaluatein vitroblood compatibility, plasma protein adsorption on the brushes was quantified with a BCA protein assay, and the adsorbed proteins on the brushes were identified using high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). All four CPBs displayed much less protein adsorption than their corresponding SDPBs. Interestingly, the number and type of identified proteins differed on the brushes. Platelet adhesion was then examined on the brushes, whereby CPBs suppressed platelet adhesion to a greater extent than the corresponding SDPBs, although platelet activation was observed on all surfaces. As a result, the CPBs of PPEGMA prevented platelet adhesion the most. After screening the polymers byin vitroevaluation, CPBs of PPEGMA were then grafted on a catheter by SI-ATRP. The catheter with the CPBs was implanted into the jugular vein of a rabbit. Thein vivoassessment after three weeks of implantation confirmed that the CPBs caused little coagulation or inflammation, whereas the pristine catheter exhibited inflammation and encapsulation.
AB - Concentrated polymer brushes (CPBs) and semi-dilute polymer brushes (SDPBs) of poly(2-hydroxyethyl methacrylate), poly(2-hydroxyethyl acrylate), poly[poly(ethylene glycol)methyl ether methacrylate] (PPEGMA) and poly(2-methoxyetyl acrylate) were prepared on silica particles and silicon wafers by surface-initiated atom transfer radical polymerization (SI-ATRP). In order to evaluatein vitroblood compatibility, plasma protein adsorption on the brushes was quantified with a BCA protein assay, and the adsorbed proteins on the brushes were identified using high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). All four CPBs displayed much less protein adsorption than their corresponding SDPBs. Interestingly, the number and type of identified proteins differed on the brushes. Platelet adhesion was then examined on the brushes, whereby CPBs suppressed platelet adhesion to a greater extent than the corresponding SDPBs, although platelet activation was observed on all surfaces. As a result, the CPBs of PPEGMA prevented platelet adhesion the most. After screening the polymers byin vitroevaluation, CPBs of PPEGMA were then grafted on a catheter by SI-ATRP. The catheter with the CPBs was implanted into the jugular vein of a rabbit. Thein vivoassessment after three weeks of implantation confirmed that the CPBs caused little coagulation or inflammation, whereas the pristine catheter exhibited inflammation and encapsulation.
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U2 - 10.1039/d1tb00886b
DO - 10.1039/d1tb00886b
M3 - Article
C2 - 34124738
AN - SCOPUS:85111446326
SN - 2050-750X
VL - 9
SP - 5794
EP - 5804
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 29
ER -