In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes

Yuki Shirosaki, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka, Maria Ascensão Lopes, José Domingos Santos, Maria Helena Fernandes

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158 Citations (Scopus)


Chitosan-silicate hybrids were synthesized using γ- glycidoxypropyltrimethoxysilane (GPSM) as the agent for cross-linking the chitosan chains. CaCl2 was introduced in the hybrids in expectation that it would improve cell adhesion and differentiation of the hybrid surfaces. Fourier-transform infrared (FT-IR) spectroscopy and 29Si CP-MAS NMR spectroscopy were used to analyze the structures of the hybrids. Cytocompatibility of the hybrids was investigated in terms of proliferation of an osteoblastic cell line, MG63. The adhesion and proliferation of the osteoblastic cells cultured on the surface of a chitosan-GPSM hybrid without calcium were similar to those on a control culture plate, and were better than those on a chitosan membrane. The ALP activity of the cells cultured on this hybrid was higher than that on the chitosan membrane. Contrary to expectations, the incorporation of calcium ions into the hybrids did not improve cell attachment and proliferation on their surfaces.

Original languageEnglish
Pages (from-to)485-493
Number of pages9
Issue number5
Publication statusPublished - Feb 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Mechanics of Materials
  • Ceramics and Composites
  • Bioengineering
  • Biophysics
  • Biomaterials


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