In situ observation of spherical DNA assembly in water and the controlled release of bound dyes

Kazunori Matsuura, Kouzo Masumoto, Yuuko Igami, Tatsuro Fujioka, Nobuo Kimizuka

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)


    Three strands of 30-mer oligodeoxyribonucleotides (ODNs) were designed to form three-way junctions that possess self-complementary sticky ends. The morphology of self-assembled ODNs in water was observed in situ by confocal laser scanning fluorescence microscopy. The three-way junctions self-assembled into spherical assemblies, in accordance with transmission and scanning electron microscopy. The size of nucleospheres was in the range of several tens of nanometers to micrometers, which varied depending on the concentration of ODNs and added salts. Fluorescence images of spherical ODN assemblies suggested that the nucleospheres possess multiwalled structures. The fluorescence of sodium 1-anilinonaphthalene-8-sulfonate in the presence of nucleospheres revealed that the interior of nucleospheres possesses polarity corresponding to that between methanol and ethanol. A dye-inclusion experiment showed that cationic and even anionic dyes were adsorbed to the interior of the nucleospheres. The dye-included nucleospheres released dyes by thermal dissociation or digestion of the constituent ODNs.

    Original languageEnglish
    Pages (from-to)2726-2732
    Number of pages7
    Issue number9
    Publication statusPublished - Sept 2007

    All Science Journal Classification (ASJC) codes

    • Bioengineering
    • Biomaterials
    • Polymers and Plastics
    • Materials Chemistry


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