In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device

Do Hyeon Yang; Ah Hyun Bae; Kazuya Koumoto; Seung Woo Lee; Kazuo Sakurai; Seiji Shinkai

doi:10.1016/j.snb.2004.07.008

In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device

Do Hyeon Yang, Ah Hyun Bae, Kazuya Koumoto, Seung Woo Lee, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Schizophyllan belongs to the β-(1 → 3)-d-glucan family and is well known as a triple helix polysaccharide. It is already known that the single chain of schizophyllan (s-SPG) can specifically form a complex with particular polynucleotides. As an application of this recognition capability, a novel schizophyllan derivative connected with a disulfide bond through a long methylene spacer was synthesized, and its interaction with polynucleotides was directly monitored with a self-assembled monolayer on QCM. The gold-coated QCM electrode was covered in ca. 60% as a monolayer of s-SPG. The QCM resonator showed sensitive mass increases for poly(C) and poly(U) in the presence of K⁺ ion, which are consistent with our previous results. The present results indicate that the schizophyllan-immobilized QCM resonator can be a new sensing device for biomolecules.

Original language	English
Pages (from-to)	490-494
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	105
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2004.07.008
Publication status	Published - Mar 28 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2004.07.008

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title = "In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device",

abstract = "Schizophyllan belongs to the β-(1 → 3)-d-glucan family and is well known as a triple helix polysaccharide. It is already known that the single chain of schizophyllan (s-SPG) can specifically form a complex with particular polynucleotides. As an application of this recognition capability, a novel schizophyllan derivative connected with a disulfide bond through a long methylene spacer was synthesized, and its interaction with polynucleotides was directly monitored with a self-assembled monolayer on QCM. The gold-coated QCM electrode was covered in ca. 60% as a monolayer of s-SPG. The QCM resonator showed sensitive mass increases for poly(C) and poly(U) in the presence of K+ ion, which are consistent with our previous results. The present results indicate that the schizophyllan-immobilized QCM resonator can be a new sensing device for biomolecules.",

author = "Yang, {Do Hyeon} and Bae, {Ah Hyun} and Kazuya Koumoto and Lee, {Seung Woo} and Kazuo Sakurai and Seiji Shinkai",

note = "Funding Information: We thank Taito Corporation for kindly providing the schizophyllan sample. This work has been financially supported by SORST program of Japan Science and Technology Corporation. ",

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doi = "10.1016/j.snb.2004.07.008",

language = "English",

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T1 - In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device

AU - Yang, Do Hyeon

AU - Bae, Ah Hyun

AU - Koumoto, Kazuya

AU - Lee, Seung Woo

AU - Sakurai, Kazuo

AU - Shinkai, Seiji

N1 - Funding Information: We thank Taito Corporation for kindly providing the schizophyllan sample. This work has been financially supported by SORST program of Japan Science and Technology Corporation.

PY - 2005/3/28

Y1 - 2005/3/28

N2 - Schizophyllan belongs to the β-(1 → 3)-d-glucan family and is well known as a triple helix polysaccharide. It is already known that the single chain of schizophyllan (s-SPG) can specifically form a complex with particular polynucleotides. As an application of this recognition capability, a novel schizophyllan derivative connected with a disulfide bond through a long methylene spacer was synthesized, and its interaction with polynucleotides was directly monitored with a self-assembled monolayer on QCM. The gold-coated QCM electrode was covered in ca. 60% as a monolayer of s-SPG. The QCM resonator showed sensitive mass increases for poly(C) and poly(U) in the presence of K+ ion, which are consistent with our previous results. The present results indicate that the schizophyllan-immobilized QCM resonator can be a new sensing device for biomolecules.

AB - Schizophyllan belongs to the β-(1 → 3)-d-glucan family and is well known as a triple helix polysaccharide. It is already known that the single chain of schizophyllan (s-SPG) can specifically form a complex with particular polynucleotides. As an application of this recognition capability, a novel schizophyllan derivative connected with a disulfide bond through a long methylene spacer was synthesized, and its interaction with polynucleotides was directly monitored with a self-assembled monolayer on QCM. The gold-coated QCM electrode was covered in ca. 60% as a monolayer of s-SPG. The QCM resonator showed sensitive mass increases for poly(C) and poly(U) in the presence of K+ ion, which are consistent with our previous results. The present results indicate that the schizophyllan-immobilized QCM resonator can be a new sensing device for biomolecules.

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

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ER -

In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device

Abstract

All Science Journal Classification (ASJC) codes

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