In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging

Yoon Hee Kim; Yoshinori Fujimura; Masako Sasaki; Xue Yang; Daichi Yukihira; Daisuke Miura; Yumi Unno; Koretsugu Ogata; Hiroki Nakajima; Shuya Yamashita; Kanami Nakahara; Motoki Murata; I. Chian Lin; Hiroyuki Wariishi; Koji Yamada; Hirofumi Tachibana

doi:10.1021/jf503143g

In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging

Yoon Hee Kim, Yoshinori Fujimura, Masako Sasaki, Xue Yang, Daichi Yukihira, Daisuke Miura, Yumi Unno, Koretsugu Ogata, Hiroki Nakajima, Shuya Yamashita, Kanami Nakahara, Motoki Murata, I. Chian Lin, Hiroyuki Wariishi, Koji Yamada, Hirofumi Tachibana

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Abstract

Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.

Original language	English
Pages (from-to)	9279-9285
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	62
Issue number	38
DOIs	https://doi.org/10.1021/jf503143g
Publication status	Published - Sept 24 2014

All Science Journal Classification (ASJC) codes

Chemistry(all)
Agricultural and Biological Sciences(all)

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Kim, Y. H., Fujimura, Y., Sasaki, M., Yang, X., Yukihira, D., Miura, D., Unno, Y., Ogata, K., Nakajima, H., Yamashita, S., Nakahara, K., Murata, M., Lin, I. C., Wariishi, H., Yamada, K., & Tachibana, H. (2014). In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging. Journal of Agricultural and Food Chemistry, 62(38), 9279-9285. https://doi.org/10.1021/jf503143g

Kim, YH, Fujimura, Y, Sasaki, M, Yang, X, Yukihira, D, Miura, D, Unno, Y, Ogata, K, Nakajima, H, Yamashita, S, Nakahara, K, Murata, M, Lin, IC, Wariishi, H, Yamada, K & Tachibana, H 2014, 'In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging', Journal of Agricultural and Food Chemistry, vol. 62, no. 38, pp. 9279-9285. https://doi.org/10.1021/jf503143g

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title = "In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging",

abstract = "Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.",

author = "Kim, {Yoon Hee} and Yoshinori Fujimura and Masako Sasaki and Xue Yang and Daichi Yukihira and Daisuke Miura and Yumi Unno and Koretsugu Ogata and Hiroki Nakajima and Shuya Yamashita and Kanami Nakahara and Motoki Murata and Lin, {I. Chian} and Hiroyuki Wariishi and Koji Yamada and Hirofumi Tachibana",

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doi = "10.1021/jf503143g",

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T1 - In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging

AU - Kim, Yoon Hee

AU - Fujimura, Yoshinori

AU - Sasaki, Masako

AU - Yang, Xue

AU - Yukihira, Daichi

AU - Miura, Daisuke

AU - Unno, Yumi

AU - Ogata, Koretsugu

AU - Nakajima, Hiroki

AU - Yamashita, Shuya

AU - Nakahara, Kanami

AU - Murata, Motoki

AU - Lin, I. Chian

AU - Wariishi, Hiroyuki

AU - Yamada, Koji

AU - Tachibana, Hirofumi

PY - 2014/9/24

Y1 - 2014/9/24

N2 - Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.

AB - Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.

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In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging

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