IN-SITU INJECTION OF MOLECULES INTO CELLS

Delivering nanoartifacts such as molecules and nanosensors into cells is a fundamental technology contributing to not only gene editing for obtaining desired cells but also measurement of intracellular environment. A method enabling to locally deliver molecules into targeted cells under culture condition is coveted, however, existing technologies represented by electroporation are hard to offer such opportunities. Here, we present an in-situ injection system based on local electroporation by a robotic-controlled microelectrode. We demonstrated two diverse applications: 2D drawing using plasmid DNA (4.7 kbp) and FITC-labeled dextran (250 kDa and 2 MDa), and in-situ injection of ribonucleoprotein (guide RNA with Cas9).