In-cell covalent labeling of reactive his-tag fused proteins

Shohei Uchinomiya; Hiroshi Nonaka; Sho Wakayama; Akio Ojida; Itaru Hamachi

doi:10.1039/c3cc41979g

In-cell covalent labeling of reactive his-tag fused proteins

Shohei Uchinomiya, Hiroshi Nonaka, Sho Wakayama, Akio Ojida, Itaru Hamachi

Molecular Bioinformatics

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(ii)-NTA containing an α-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein-protein interaction was achieved inside living cells.

Original language	English
Pages (from-to)	5022-5024
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	44
DOIs	https://doi.org/10.1039/c3cc41979g
Publication status	Published - May 7 2013

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c3cc41979g

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abstract = "A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(ii)-NTA containing an α-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein-protein interaction was achieved inside living cells.",

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AU - Uchinomiya, Shohei

AU - Nonaka, Hiroshi

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AU - Ojida, Akio

AU - Hamachi, Itaru

PY - 2013/5/7

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AB - A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(ii)-NTA containing an α-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein-protein interaction was achieved inside living cells.

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In-cell covalent labeling of reactive his-tag fused proteins

Abstract

All Science Journal Classification (ASJC) codes

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