Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae

Hiroshi Koyama; Eriko Sumiya; Takahiro Ito; Kazuhisa Sekimizu

doi:10.1111/j.1567-1364.2007.00334.x

Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae

Hiroshi Koyama, Eriko Sumiya, Takahiro Ito, Kazuhisa Sekimizu

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The PCR-based gene disruption strategy originally devised by Baudin et al. is widely used for gene targeting in Saccharomyces cerevisiae. An advantage of this strategy is its simplicity in making targeting constructs. The efficiencies of the targeted disruption are highly variable from locus to locus, however, and often very low. In this report, a method for improving the gene deletion efficiency is described.

Original language	English
Pages (from-to)	193-194
Number of pages	2
Journal	FEMS Yeast Research
Volume	8
Issue number	2
DOIs	https://doi.org/10.1111/j.1567-1364.2007.00334.x
Publication status	Published - Mar 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Applied Microbiology and Biotechnology

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10.1111/j.1567-1364.2007.00334.x

Cite this

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abstract = "The PCR-based gene disruption strategy originally devised by Baudin et al. is widely used for gene targeting in Saccharomyces cerevisiae. An advantage of this strategy is its simplicity in making targeting constructs. The efficiencies of the targeted disruption are highly variable from locus to locus, however, and often very low. In this report, a method for improving the gene deletion efficiency is described.",

author = "Hiroshi Koyama and Eriko Sumiya and Takahiro Ito and Kazuhisa Sekimizu",

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Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae

Abstract

All Science Journal Classification (ASJC) codes

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