Improved brilliant blue G staining of the internal limiting membrane with sharp cut filters of a novel viewing filter system

Hiroshi Enaida, Shigeo Yoshida, Shintaro Nakao, Yasuhiro Ikeda, Yoshiyuki Hachisuka, Yusuke Oshima, Kazuaki Kadonosono, Akifumi Ueno, Tatsuro Ishibashi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Purpose: We developed a new artificial image enhancement system aimed at intraoperative visibility improvement as a clinical prototype. We examined each optical characteristic and change in intraoperative visibility using brilliant blue G (BBG) staining with various sharp cut filters (SCFs). Method: This was a retrospective and observational study. The system was composed of several filters attached to the operating microscope. Six eyes from 6 patients who presented with macular hole and underwent surgery using this system were studied. As a clinical examination, the intraoperative visibility of BBG staining intensities was compared for 4 kinds of SCFs during vitrectomy. Quantitative evaluation was calculated using the International Commission on Illumination 1976 (L∗, a∗, b∗) color space (CIELAB) method. Furthermore, we evaluated each optical characteristic of 4 types of SCFs using extracted porcine eyes and a spectroradiometer as a clinical simulation. Results: Suitable filter selection was possible for this system. The observed color tone and spectral irradiance changes with SCF insertion changed dynamically. In macular hole cases, the color intensities between BBG-stained and nonstained areas were improved using SCF-455 and SCF-520, which was statistically significant (p < 0.05) by CIELAB. Conclusion: The system improved BBG staining intensity with the use of selective SCFs.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
Publication statusPublished - Apr 18 2013

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems


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