TY - JOUR
T1 - Imprinted genes are up-regulated by growth arrest in embryonic fibroblasts
AU - Hayashida, Toshiro
AU - Eversole-Cire, Pamela
AU - Jones, Peter A.
AU - Sasaki, Hiroyuki
PY - 1997/11
Y1 - 1997/11
N2 - It was previously reported that the imprinted insulin-like growth factor II and H19 genes are up-regulated in embryonic fibroblasts upon growth arrest. We have examined six other imprinted genes and found that all were up-regulated upon confluency and at least four of them by serum starvation. The significance of the results was confirmed by demonstrating that only one-third of randomly selected genes are up-regulated upon confluency. Our findings suggest that the cell cycle should be carefully controlled when imprinted genes are studied in cell cultures. Moreover, the unique property could have significance in the mechanistic or the evolutionary aspect of imprinting or both.
AB - It was previously reported that the imprinted insulin-like growth factor II and H19 genes are up-regulated in embryonic fibroblasts upon growth arrest. We have examined six other imprinted genes and found that all were up-regulated upon confluency and at least four of them by serum starvation. The significance of the results was confirmed by demonstrating that only one-third of randomly selected genes are up-regulated upon confluency. Our findings suggest that the cell cycle should be carefully controlled when imprinted genes are studied in cell cultures. Moreover, the unique property could have significance in the mechanistic or the evolutionary aspect of imprinting or both.
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U2 - 10.1093/oxfordjournals.jbchem.a021850
DO - 10.1093/oxfordjournals.jbchem.a021850
M3 - Article
C2 - 9443803
AN - SCOPUS:0030727933
SN - 0021-924X
VL - 122
SP - 901
EP - 903
JO - Journal of biochemistry
JF - Journal of biochemistry
IS - 5
ER -