TY - JOUR
T1 - Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics
AU - Takeshita, Minoru
AU - Matsuo, Yuka
AU - Suzuki, Masashi
AU - Furuya, Naruto
AU - Tsuchiya, Kenichi
AU - Takanami, Yoichi
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research 14760029 and 21780040 from the Ministry of Education, Culture, Sports, Science and Technology-Japan.
PY - 2009
Y1 - 2009
N2 - D RNA 3Yα (D3Yα), a defective (D) RNA 3 derived from the Y strain of cucumber mosaic virus (CMV-Y), was further characterized in combination with different helper viruses in the genus Cucumovirus. Interestingly, Nicotiana benthamiana plants inoculated with CMV-D8 and D3Yα developed systemic symptoms which were different from those induced by CMV-D8. To elucidate the potential effects of D RNA 3 on virus infection on the basis of the original combination of CMV-Y and D3Yα, a point mutation was made in the coat protein gene, which determined symptoms, of either CMV-Y RNA 3 (Y3) or D3Yα. Symptoms induced on N. benthamiana and N. tabacum plants, and semi-quantitative RT-PCR revealed that the ratio of RNA 3 to D RNA 3 was associated with the differences of symptoms in the leaf tissues. Furthermore, analysis of in situ hybridization suggested that there were spatial effects between coat proteins of Y3 and D3Yα in the infected leaves.
AB - D RNA 3Yα (D3Yα), a defective (D) RNA 3 derived from the Y strain of cucumber mosaic virus (CMV-Y), was further characterized in combination with different helper viruses in the genus Cucumovirus. Interestingly, Nicotiana benthamiana plants inoculated with CMV-D8 and D3Yα developed systemic symptoms which were different from those induced by CMV-D8. To elucidate the potential effects of D RNA 3 on virus infection on the basis of the original combination of CMV-Y and D3Yα, a point mutation was made in the coat protein gene, which determined symptoms, of either CMV-Y RNA 3 (Y3) or D3Yα. Symptoms induced on N. benthamiana and N. tabacum plants, and semi-quantitative RT-PCR revealed that the ratio of RNA 3 to D RNA 3 was associated with the differences of symptoms in the leaf tissues. Furthermore, analysis of in situ hybridization suggested that there were spatial effects between coat proteins of Y3 and D3Yα in the infected leaves.
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U2 - 10.1016/j.virol.2009.04.010
DO - 10.1016/j.virol.2009.04.010
M3 - Article
C2 - 19427011
AN - SCOPUS:67349282267
SN - 0042-6822
VL - 389
SP - 59
EP - 65
JO - Virology
JF - Virology
IS - 1-2
ER -