Immunohistochemical localization of d-alanine to β-cells in rat pancreas

Akiko Morikawa, Kenji Hamase, Tomohiro Ohgusu, Sachise Etoh, Hiroyuki Tanaka, Ichiro Koshiishi, Yukihiro Shoyama, Kiyoshi Zaitsu

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50 Citations (Scopus)


A mouse monoclonal antibody against d-alanine (d-Ala) has been raised and the immunohistochemical localization of this d-amino acids in the rat pancreas is visualized. The obtained anti-d-Ala monoclonal antibody has no significant cross-reactivity to all proteinogenic l-amino acids and their d-enantiomers. Using this antibody, immunohistochemical staining was performed on the pancreas, and specific staining for d-Ala has been observed only in the Langerhans islets. To identify the types of d-Ala-immunopositive cells, double staining was carried out with antibodies against d-Ala and pancreatic hormones. Similar immunostaining patterns have been observed for d-Ala and insulin, while d-Ala is hardly co-localized with other hormones (glucagon, somatostatin, and pancreatic polypeptide). These results indicate for the first time that d-Ala is localized to insulin producing β-cells in mammalian pancreas, suggesting that this d-amino acid would be involved in the regulation of the blood glucose level.

Original languageEnglish
Pages (from-to)872-876
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number4
Publication statusPublished - Apr 20 2007

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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