Obstructive sialoadenitis was examined by immunohistochemical techniques for keratin (MoAb K.LI, PKK.I and K.8.12) and actin. Electronmicroscopy (EMS) was used to identify ultrastructural changes in myoepithelial cells and ductal basal cells. With immunohistochemistry, actin staining was used as a marker of myoepithelium. MoAbs KL1 and PKK1 for ductal luminal cells, and MoAb K8.12 for ductal basal cells. Histologic features of the lesion usually showed degenerative changes of acinar and duct cells with cell infiltration and fibrous replacement. Immunohistochemical findings indicated that actin staining in the changed myoepithelial cells was irregularly positive or negative, and also keratin staining in luminal and ductal basal cells was reduced or disappeared. Ultra‐structural features of the changed myoepithelial cells indicated that these cells appeared less altered than adjacent acinar and ductal cells and showed increased amounts of lipid droplets and lipofuscin granules, and also wrinkled processes filled the prominent myofilamenl material.
|Number of pages||6|
|Journal||Journal of Oral Pathology & Medicine|
|Publication status||Published - Sept 1992|
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Oral Surgery
- Cancer Research