Immunocytochemical identification of Pit-1 containing cells in the anterior pituitary of hens

Vishwajit S. Chowdhury, Yukinori Yoshimura

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3 Citations (Scopus)


Our goal was to identify the cells expressing Pit-1 protein in chicken anterior pituitary. The anterior pituitaries were collected from laying hens after perfusion with formalin-PBS, and fixed with Bouin's fixative followed by paraffin embedding. Sections of the anterior pituitaries were immunostained for Pit-1 in the first staining sequence followed by staining for 6 types of pituitary hormones in the second sequence. Pit-1 positive nuclei were observed in the glandular cells in both the cephalic and caudal lobes. Pit-1 immunoreaction products were colocalized in the glandular cells immunopositive for growth hormone, thyroid-stimulating hormone, follicle-stimulating hormone, luteinizing hormone, adrenocorticotropic hormone or prolactin. These results indicate that Pit-1 protein induction occurs in 6 types of glandular cells, suggesting that Pit-1 may regulate hormone synthesis in each glandular cell in the chicken pituitary.

Original languageEnglish
Pages (from-to)375-379
Number of pages5
JournalJournal of Reproduction and Development
Issue number5
Publication statusPublished - Dec 1 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology


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