TY - JOUR
T1 - Immunochromatographic strip assay for detection of bioactive Ganoderma triterpenoid, ganoderic acid A in Ganoderma lingzhi
AU - Sakamoto, Seiichi
AU - Kikkawa, Nao
AU - Kohno, Toshitaka
AU - Shimizu, Kuniyoshi
AU - Tanaka, Hiroyuki
AU - Morimoto, Satoshi
N1 - Funding Information:
We appreciate Ken Sawai and Takeshi Sawai (Toyotanshien Co Ltd., Sapporo, Japan) providing G. lingzhi used in this study. This work was supported by a Grant-in-Aid for challenging Exploratory Research [ 26660147 ] of the Japan Society for the Promotion of Science (JSPS).
Publisher Copyright:
© 2016
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Ganoderic acid A (GAA) is one of the major Ganoderma triterpenes produced by medicinal mushroom belonging to the genus Ganoderma (Ganodermataceae). Due to its interesting pharmacological activities, Ganoderma species have been traditionally used in China for the treatment of various diseases. Herein, we developed a colloidal gold-based immunochromatographic strip assay (ICA) for the rapid detection of GAA using highly specific monoclonal antibody against GAA (MAb 12A) conjugated with gold nanoparticles. Using the developed ICA, the detection of GAA can be completed within 15 min after dipping the test strip into an analyte solution with the limit of detection (LOD) for GAA of ~ 500 ng/mL. In addition, this system makes it possible to perform a semi-quantitative analysis of GAA in Ganoderma lingzhi, where high reliability was evaluated by enzyme-linked immunosorbent assay (ELISA). The newly developed ICA can potentially be applied to the standardization of Ganoderma using GAA as an index because GAA is major triterpenoid present much in the mushroom.
AB - Ganoderic acid A (GAA) is one of the major Ganoderma triterpenes produced by medicinal mushroom belonging to the genus Ganoderma (Ganodermataceae). Due to its interesting pharmacological activities, Ganoderma species have been traditionally used in China for the treatment of various diseases. Herein, we developed a colloidal gold-based immunochromatographic strip assay (ICA) for the rapid detection of GAA using highly specific monoclonal antibody against GAA (MAb 12A) conjugated with gold nanoparticles. Using the developed ICA, the detection of GAA can be completed within 15 min after dipping the test strip into an analyte solution with the limit of detection (LOD) for GAA of ~ 500 ng/mL. In addition, this system makes it possible to perform a semi-quantitative analysis of GAA in Ganoderma lingzhi, where high reliability was evaluated by enzyme-linked immunosorbent assay (ELISA). The newly developed ICA can potentially be applied to the standardization of Ganoderma using GAA as an index because GAA is major triterpenoid present much in the mushroom.
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U2 - 10.1016/j.fitote.2016.08.016
DO - 10.1016/j.fitote.2016.08.016
M3 - Article
C2 - 27575328
AN - SCOPUS:84984636003
SN - 0367-326X
VL - 114
SP - 51
EP - 55
JO - Fitoterapia
JF - Fitoterapia
ER -