Immunochemical detection of methamphetamine using polymer-protected ultrafine platinum particles

Emi Hifumi; Rie Kanmei; Masanori Ishimaru; Kosuke Shimizu; Michitaka Ohtaki; Taizo Uda

doi:10.1016/0922-338X(96)89164-1

Immunochemical detection of methamphetamine using polymer-protected ultrafine platinum particles

Emi Hifumi, Rie Kanmei, Masanori Ishimaru, Kosuke Shimizu, Michitaka Ohtaki, Taizo Uda

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Ultrafine platinum particles protected by poly (methyl acrylate-co-N- vinyl-2-pyrrolidone) were applied to the immunological detection of methamphetamine (MA). The polymer-protected ultrafine particles could chemically bind anti-methamphetamine monoclonal antibody to their surfaces. The antibody-fixed particles behaved like an antibody in the immunoreaction. The antibody-bound particles competitively reacted against the immobilized and the free antigens in the ELISA system. MA could be detected down to a concentration of ca. 10 ng/ml. In the immuno-dot assay, 10-100 ng/ml of MA was detected within 30 min using the ultrafine particles.

Original language	English
Pages (from-to)	417-419
Number of pages	3
Journal	Journal of Fermentation and Bioengineering
Volume	82
Issue number	4
DOIs	https://doi.org/10.1016/0922-338X(96)89164-1
Publication status	Published - 1996

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology

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10.1016/0922-338X(96)89164-1

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title = "Immunochemical detection of methamphetamine using polymer-protected ultrafine platinum particles",

abstract = "Ultrafine platinum particles protected by poly (methyl acrylate-co-N- vinyl-2-pyrrolidone) were applied to the immunological detection of methamphetamine (MA). The polymer-protected ultrafine particles could chemically bind anti-methamphetamine monoclonal antibody to their surfaces. The antibody-fixed particles behaved like an antibody in the immunoreaction. The antibody-bound particles competitively reacted against the immobilized and the free antigens in the ELISA system. MA could be detected down to a concentration of ca. 10 ng/ml. In the immuno-dot assay, 10-100 ng/ml of MA was detected within 30 min using the ultrafine particles.",

author = "Emi Hifumi and Rie Kanmei and Masanori Ishimaru and Kosuke Shimizu and Michitaka Ohtaki and Taizo Uda",

note = "Funding Information: This study has been supportedi n part by the Yazaki Memorial Foundation for Science& Technology.",

year = "1996",

doi = "10.1016/0922-338X(96)89164-1",

language = "English",

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pages = "417--419",

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T1 - Immunochemical detection of methamphetamine using polymer-protected ultrafine platinum particles

AU - Hifumi, Emi

AU - Kanmei, Rie

AU - Ishimaru, Masanori

AU - Shimizu, Kosuke

AU - Ohtaki, Michitaka

AU - Uda, Taizo

N1 - Funding Information: This study has been supportedi n part by the Yazaki Memorial Foundation for Science& Technology.

PY - 1996

Y1 - 1996

N2 - Ultrafine platinum particles protected by poly (methyl acrylate-co-N- vinyl-2-pyrrolidone) were applied to the immunological detection of methamphetamine (MA). The polymer-protected ultrafine particles could chemically bind anti-methamphetamine monoclonal antibody to their surfaces. The antibody-fixed particles behaved like an antibody in the immunoreaction. The antibody-bound particles competitively reacted against the immobilized and the free antigens in the ELISA system. MA could be detected down to a concentration of ca. 10 ng/ml. In the immuno-dot assay, 10-100 ng/ml of MA was detected within 30 min using the ultrafine particles.

AB - Ultrafine platinum particles protected by poly (methyl acrylate-co-N- vinyl-2-pyrrolidone) were applied to the immunological detection of methamphetamine (MA). The polymer-protected ultrafine particles could chemically bind anti-methamphetamine monoclonal antibody to their surfaces. The antibody-fixed particles behaved like an antibody in the immunoreaction. The antibody-bound particles competitively reacted against the immobilized and the free antigens in the ELISA system. MA could be detected down to a concentration of ca. 10 ng/ml. In the immuno-dot assay, 10-100 ng/ml of MA was detected within 30 min using the ultrafine particles.

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JO - Journal of Fermentation and Bioengineering

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Immunochemical detection of methamphetamine using polymer-protected ultrafine platinum particles

Abstract

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