Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application

H. Yanagihara; R. Sakata; H. Minami; H. Tanaka; Y. Shoyama; H. Murakami

doi:10.1016/S0003-2670(96)00371-6

Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application

H. Yanagihara, R. Sakata, H. Minami, H. Tanaka, Y. Shoyama, H. Murakami

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Abstract

Immunoaffinity column chromatography using anti-forskolin monoclonal antibodies appears to be far superior to previously published separation methods based on crystallization and chromatography. The capacity of the immunoaffinity column as determined by ELISA is 9 μg ml^-1. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.

Original language	English
Pages (from-to)	63-70
Number of pages	8
Journal	Analytica Chimica Acta
Volume	335
Issue number	1-2
DOIs	https://doi.org/10.1016/S0003-2670(96)00371-6
Publication status	Published - Dec 10 1996

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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abstract = "Immunoaffinity column chromatography using anti-forskolin monoclonal antibodies appears to be far superior to previously published separation methods based on crystallization and chromatography. The capacity of the immunoaffinity column as determined by ELISA is 9 μg ml-1. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.",

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AU - Sakata, R.

AU - Minami, H.

AU - Tanaka, H.

AU - Shoyama, Y.

AU - Murakami, H.

PY - 1996/12/10

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AB - Immunoaffinity column chromatography using anti-forskolin monoclonal antibodies appears to be far superior to previously published separation methods based on crystallization and chromatography. The capacity of the immunoaffinity column as determined by ELISA is 9 μg ml-1. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.

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Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application

Abstract

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