TY - GEN
T1 - Immuno-wall lab-on-a-chip protein analysis devices for high precision surgery of gliomas
AU - Kasama, T.
AU - Yamamichi, A.
AU - Ohka, F.
AU - Kato, Y.
AU - Suzuki, H.
AU - Kato, A.
AU - Motomura, K.
AU - Hirano, M.
AU - Ranjit, M.
AU - Chalise, L.
AU - Kurimoto, M.
AU - Kondo, G.
AU - Aoki, K.
AU - Kaji, N.
AU - Matsubara, T.
AU - Suzuki, H.
AU - Tokeshi, M.
AU - Wakabayashi, T.
AU - Natsume, A.
AU - Baba, Y.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016
Y1 - 2016
N2 - In the present study, we propose immuno-wall lab-on-a-chip protein analysis devices for ultrarapid detection of R132H mutant isocitrate dehydrogenase 1 (IDH1) that we identified as a specifically and commonly expressed gene in diffusely infiltrative gliomas1. In 9 min immunoassay, we successfully carried out specific detection of R132H mutant IDH1 in a lysate of glioma tissue, which is much faster than intraoperative pathological diagnosis. Moreover, clinical samples from 10 patients with gliomas were analyzed by using the immuno-wall devices. These results were consistent with those of PCR-based genetic mutation detection method. Our devices realize point-of-care tumor testing, resulting in the high precision surgery of gliomas.
AB - In the present study, we propose immuno-wall lab-on-a-chip protein analysis devices for ultrarapid detection of R132H mutant isocitrate dehydrogenase 1 (IDH1) that we identified as a specifically and commonly expressed gene in diffusely infiltrative gliomas1. In 9 min immunoassay, we successfully carried out specific detection of R132H mutant IDH1 in a lysate of glioma tissue, which is much faster than intraoperative pathological diagnosis. Moreover, clinical samples from 10 patients with gliomas were analyzed by using the immuno-wall devices. These results were consistent with those of PCR-based genetic mutation detection method. Our devices realize point-of-care tumor testing, resulting in the high precision surgery of gliomas.
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M3 - Conference contribution
AN - SCOPUS:85014200346
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 651
EP - 652
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -