TY - GEN
T1 - Immuno-chromatographic test-strip for skin cathepsin L analysis
AU - Yamaguchi, M.
AU - Kosaka, S.
AU - Tahara, Y.
AU - Date, A.
PY - 2009
Y1 - 2009
N2 - The objective is to develop a methodology to analyze cathepsin L (Cat L) activity derived from the stratum corneum as an index of skin conditions. In this paper, a disposable type immuno-chromatographic test-strip (test-strip) is proposed for Cat L analysis. A fabricated test-strip for Cat L analysis consists of a sample pad, a conjugate pad, a nitrocellulose membrane and absorption pad. After dropping a sample, it was needed 90 s to reach the solution at the test-line on the test-strip. Three min after dropping, a reddish purple colored band was appeared as the test-line. The band visually observed clearly when the concentration of Cat L of the standard solution over 0.01 U/l. It was indicated a possibility of the disposable type immuno-chromatographic test-strip for the analysis of Cat L of skin.
AB - The objective is to develop a methodology to analyze cathepsin L (Cat L) activity derived from the stratum corneum as an index of skin conditions. In this paper, a disposable type immuno-chromatographic test-strip (test-strip) is proposed for Cat L analysis. A fabricated test-strip for Cat L analysis consists of a sample pad, a conjugate pad, a nitrocellulose membrane and absorption pad. After dropping a sample, it was needed 90 s to reach the solution at the test-line on the test-strip. Three min after dropping, a reddish purple colored band was appeared as the test-line. The band visually observed clearly when the concentration of Cat L of the standard solution over 0.01 U/l. It was indicated a possibility of the disposable type immuno-chromatographic test-strip for the analysis of Cat L of skin.
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U2 - 10.1149/1.2981106
DO - 10.1149/1.2981106
M3 - Conference contribution
AN - SCOPUS:63849316359
SN - 9781566776578
T3 - ECS Transactions
SP - 67
EP - 73
BT - ECS Transactions - Chemical Sensors 8
PB - Electrochemical Society Inc.
T2 - Chemical Sensors 8: Chemical (Gas, Ion, Bio) Sensors and Analytical Systems - 214th ECS Meeting
Y2 - 12 October 2008 through 17 October 2008
ER -