Immobilization of surfactant-lipase complexes and their high heat resistance in organic media

Muneharu Goto; Chiaki Hatanaka; Masahiro Goto

doi:10.1016/j.bej.2005.01.027

Immobilization of surfactant-lipase complexes and their high heat resistance in organic media

Muneharu Goto, Chiaki Hatanaka, Masahiro Goto

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Surfactant-lipase complexes were immobilized in an n-vinyl-2-pyrrolidone gel matrix. Features of a native lipase and gel-immobilized surfactant-lipase complexes were measured by esterification reaction between lauric acid and benzyl alcohol in isooctane. Optimal gel-immobilized surfactant-lipase complex activity was 37.2 mol h^-1 kg^-1-lipase. Gel-immobilized lipase complexes showed a 51-fold increase in activity and exhibited superior heat resistance compared to native lipases. The optimum temperature for the immobilized lipase complex was 60°C, as compared to 37°C for native lipase activity. Gel-immobilized lipase complexes could be readily recovered, and their high activity was completely preserved, even after 10 reuses.

Original language	English
Pages (from-to)	91-94
Number of pages	4
Journal	Biochemical Engineering Journal
Volume	24
Issue number	1
DOIs	https://doi.org/10.1016/j.bej.2005.01.027
Publication status	Published - May 15 2005

All Science Journal Classification (ASJC) codes

Bioengineering
Biotechnology
Environmental Engineering
Biomedical Engineering

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10.1016/j.bej.2005.01.027

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abstract = "Surfactant-lipase complexes were immobilized in an n-vinyl-2-pyrrolidone gel matrix. Features of a native lipase and gel-immobilized surfactant-lipase complexes were measured by esterification reaction between lauric acid and benzyl alcohol in isooctane. Optimal gel-immobilized surfactant-lipase complex activity was 37.2 mol h-1 kg-1-lipase. Gel-immobilized lipase complexes showed a 51-fold increase in activity and exhibited superior heat resistance compared to native lipases. The optimum temperature for the immobilized lipase complex was 60°C, as compared to 37°C for native lipase activity. Gel-immobilized lipase complexes could be readily recovered, and their high activity was completely preserved, even after 10 reuses.",

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Immobilization of surfactant-lipase complexes and their high heat resistance in organic media

Abstract

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