Immobilization of bacterial luciferase into poly(n-isopropylacrylamide) film for electrochemical control of a bioluminescence reaction

Yoji Kawanami; Shinya Yamasaki; Shuto Yamada; Kô Takehara

doi:10.2116/analsci.28.1013

Immobilization of bacterial luciferase into poly(n-isopropylacrylamide) film for electrochemical control of a bioluminescence reaction

Yoji Kawanami, Shinya Yamasaki, Shuto Yamada, Kô Takehara

Inorganic and Analytical Chemistry

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A poly(N-isopropylacrylamide) film was modified on an indium-tin oxide electrode in order to immobilize bacterial luciferase (BL) on the electrode surface. By using the modified electrode, flavin mononucleotide (FMN) was electrochemically reduced to FMNH2, which is one of the substrates of the BL luminescence reaction, to control the bioluminescence reaction by BL. The BL reaction in the modified film could be promoted and controlled by the electrochemical generation of FMNH₂. This BL luminescence system was evaluated as a model system for the inhibitory assay of hydrophobic molecules on protein functions. 2012

Original language	English
Pages (from-to)	1013-1015
Number of pages	3
Journal	analytical sciences
Volume	28
Issue number	10
DOIs	https://doi.org/10.2116/analsci.28.1013
Publication status	Published - 2012

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.2116/analsci.28.1013

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abstract = "A poly(N-isopropylacrylamide) film was modified on an indium-tin oxide electrode in order to immobilize bacterial luciferase (BL) on the electrode surface. By using the modified electrode, flavin mononucleotide (FMN) was electrochemically reduced to FMNH2, which is one of the substrates of the BL luminescence reaction, to control the bioluminescence reaction by BL. The BL reaction in the modified film could be promoted and controlled by the electrochemical generation of FMNH2. This BL luminescence system was evaluated as a model system for the inhibitory assay of hydrophobic molecules on protein functions. 2012",

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AU - Kawanami, Yoji

AU - Yamasaki, Shinya

AU - Yamada, Shuto

AU - Takehara, Kô

PY - 2012

Y1 - 2012

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AB - A poly(N-isopropylacrylamide) film was modified on an indium-tin oxide electrode in order to immobilize bacterial luciferase (BL) on the electrode surface. By using the modified electrode, flavin mononucleotide (FMN) was electrochemically reduced to FMNH2, which is one of the substrates of the BL luminescence reaction, to control the bioluminescence reaction by BL. The BL reaction in the modified film could be promoted and controlled by the electrochemical generation of FMNH2. This BL luminescence system was evaluated as a model system for the inhibitory assay of hydrophobic molecules on protein functions. 2012

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Immobilization of bacterial luciferase into poly(n-isopropylacrylamide) film for electrochemical control of a bioluminescence reaction

Abstract

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