TY - GEN
T1 - Identification of single molecular DNA methylation points by microfluidic DNA molecule stretching and quantum dot detection
AU - Okamoto, Yukihiro
AU - Sano, Tatsuki
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2012
Y1 - 2012
N2 - We report a rapid and simple epigenetic analysis based on microfluidic single DNA molecular methylation detection. We developed a microfluidic device to stretch a single DNA molecule and subsequent detection of a methylated DNA base by quantum dot (QD)-immobilized methyl binding domain (MBD) protein. A QD-MBD complex specifically bound to a methylated base of stretched DNA and was detected by a total internal reflection fluorescence microscope (TIRFM). Our method enables us to detect a number of methylation site and identify the methylated positions simultaneously in the simple and rapid manner compared with the conventional methods.
AB - We report a rapid and simple epigenetic analysis based on microfluidic single DNA molecular methylation detection. We developed a microfluidic device to stretch a single DNA molecule and subsequent detection of a methylated DNA base by quantum dot (QD)-immobilized methyl binding domain (MBD) protein. A QD-MBD complex specifically bound to a methylated base of stretched DNA and was detected by a total internal reflection fluorescence microscope (TIRFM). Our method enables us to detect a number of methylation site and identify the methylated positions simultaneously in the simple and rapid manner compared with the conventional methods.
UR - https://www.scopus.com/pages/publications/84893483885
UR - https://www.scopus.com/pages/publications/84893483885#tab=citedBy
M3 - Conference contribution
AN - SCOPUS:84893483885
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1939
EP - 1941
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -