Identification of genes differentially expressed in osteoclast-like cells

Hisayuki Nomiyama, Kimie Egami, Naohisa Wada, Kazuko Tou, Madoka Horiuchi, Hiromi Matsusaki, Retsu Miura, Osamu Yoshie, Toshio Kukita

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Homeostasis of the skeletal system is maintained by a balance between bone formation and resorption. The receptor activator of NF-κB ligand (RANKL) induces the differentiation of bone-resorbing cells, osteoclasts. To identify genes regulated during osteoclast differentiation, we constructed a subtraction cDNA library using a mouse RAW264 macrophage cell line that differentiates into osteoclast-like multinucleated cells after treatment with RANKL. Northern blot analysis showed that RANKL treatment upregulated expression of 17 genes. Among these were the genes for five H+-ATPase subunits, two chemokines, and the osteoclast marker cathepsin K. In addition, a mouse homolog of human dendritic cell (DC)-specific transmembrane protein (DC-STAMP), whose function in osteoclastogenesis was recently revealed, was also included in the induced genes. Characterization of these inducible genes will provide an insight into the biology of osteoclasts and the mechanism of bone-related diseases.

Original languageEnglish
Pages (from-to)227-231
Number of pages5
JournalJournal of Interferon and Cytokine Research
Issue number4
Publication statusPublished - Apr 2005

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology


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