Identification of fructo- and malto-oligosaccharides in cured tobacco leaves (Nicotiana tabacum)

Atsushi Nagai, Takeshi Yamamoto, Hiroyuki Wariishi

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13 Citations (Scopus)


Fructo-oligosaccharides (FOSs) and malto-oligosaccharides (MOSs) in cured tobacco leaves (Nicotiana tabacum) were detected and quantified using high-performance liquid chromatography (HPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS/MS). These oligosaccharides were present in several tobacco materials including flue-cured tobacco, sun/air-cured tobacco, and cut filler of commercially available tobacco products, but were not detected in air-cured tobacco. The changes in these oligosaccharides during storage were also investigated. The results revealed that MOSs simply decreased under a warm atmosphere and FOSs increased. In addition, the formation of FOSs in cured tobacco leaves occurred in the presence of sucrose. With regard to FOSs, it has been reported that green tobacco leaves do not contain FOSs such as kestose, nystose, and fructosyl-nystose. The results of a model test suggested that the changes in FOS amount were caused by enzymatic reactions.

Original languageEnglish
Pages (from-to)6606-6612
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number26
Publication statusPublished - Jul 4 2012

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)


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