TY - JOUR
T1 - Identification of DNA copy number aberrations associated with metastases of colorectal cancer using array CGH profiles
AU - Nakao, Motonao
AU - Kawauchi, Shigeto
AU - Furuya, Tomoko
AU - Uchiyama, Tetuji
AU - Adachi, Jun
AU - Okada, Takae
AU - Ikemoto, Kenzo
AU - Oga, Atsunori
AU - Sasaki, Kohsuke
N1 - Funding Information:
The study protocol was conducted under the approval of the Institutional Review Board for Human Use at the Yamaguchi University School of Medicine in 2004, and informed consent for this study was obtained from all patients. This study is supported by grants from the Japan Society for the Promotion of Science (19390102) and NEDO.
PY - 2009/1/15
Y1 - 2009/1/15
N2 - It is important to estimate the biological characteristics of tumors, including the nodal status at the time of diagnosis for optimal treatment of individual cancer patients. Array-based comparative genomic hybridization (aCGH) was performed on 77 sporadic colorectal adenocarcinomas using a chip spotted with 4030 BAC clones. The nodal status was compared with an aCGH profiles depicted using a combination of decision-tree classifier and a Self-Organizing Map (SOM) analysis. Node metastasis was not detected in any of the 6 poorly differentiated adenocarcinomas with a 3q loss. A SOM analysis following the decision-tree classification of the aCGH data allowed for the differentiation in chromosomal regions between high- and low-level decreases in the DNA copy number. Node metastasis was detected in all 5 tumors with the high-level decrease in DNA copy number at Xp, irrespective of the histological type. Node metastasis was also found exclusively in 6 tumors with increase in DNA copy number at the chromosomal region between 11q13.3 and 11q22.3. Copy number aberrations linked to nodal metastasis were identified more collectively by the combination of the decision-tree classifier and a SOM analysis than by the conventional analysis method in aCGH analysis.
AB - It is important to estimate the biological characteristics of tumors, including the nodal status at the time of diagnosis for optimal treatment of individual cancer patients. Array-based comparative genomic hybridization (aCGH) was performed on 77 sporadic colorectal adenocarcinomas using a chip spotted with 4030 BAC clones. The nodal status was compared with an aCGH profiles depicted using a combination of decision-tree classifier and a Self-Organizing Map (SOM) analysis. Node metastasis was not detected in any of the 6 poorly differentiated adenocarcinomas with a 3q loss. A SOM analysis following the decision-tree classification of the aCGH data allowed for the differentiation in chromosomal regions between high- and low-level decreases in the DNA copy number. Node metastasis was detected in all 5 tumors with the high-level decrease in DNA copy number at Xp, irrespective of the histological type. Node metastasis was also found exclusively in 6 tumors with increase in DNA copy number at the chromosomal region between 11q13.3 and 11q22.3. Copy number aberrations linked to nodal metastasis were identified more collectively by the combination of the decision-tree classifier and a SOM analysis than by the conventional analysis method in aCGH analysis.
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U2 - 10.1016/j.cancergencyto.2008.09.013
DO - 10.1016/j.cancergencyto.2008.09.013
M3 - Article
C2 - 19100508
AN - SCOPUS:57549099638
SN - 0165-4608
VL - 188
SP - 70
EP - 76
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 2
ER -
