Identification of distinct ligands for the C-type lectin receptors mincle and dectin-2 in the pathogenic fungus Malassezia

Tetsuaki Ishikawa, Fumie Itoh, Sayumi Yoshida, Shinobu Saijo, Tetsuhiro Matsuzawa, Tohru Gonoi, Takashi Saito, Yoshio Okawa, Nobuyuki Shibata, Tomofumi Miyamoto, Sho Yamasaki

Research output: Contribution to journalArticlepeer-review

167 Citations (Scopus)


Various C-type lectin receptors (CLRs), including Mincle and Dectin-2, function as pattern recognition receptors and play a central role in immunity to fungal pathogens. However, the precise structures of the CLR ligands in various pathogenic fungi have yet to be completely defined. Here we report that Malassezia, an opportunistic skin fungal pathogen, is cooperatively recognized by Mincle and Dectin-2 through distinct ligands. Solvent-based fractionation revealed that Mincle and Dectin-2 recognize lipophilic and hydrophilic components of Malassezia, respectively. Mass spectrometry and nuclear magnetic resonance (NMR) revealed glyceroglycolipid and unique mannosyl fatty acids linked to mannitol as two Mincle ligands. An O-linked mannobiose-rich glycoprotein was identified as a Malassezia ligand for Dectin-2. Cytokine production in response to the Mincle ligands and the Dectin-2 ligand was abrogated in Mincle-/- and Dectin-2-/- dendritic cells, respectively. These results demonstrate that Mincle and Dectin-2 recognize distinct ligands in Malassezia to induce host immune responses.

Original languageEnglish
Pages (from-to)477-488
Number of pages12
JournalCell Host and Microbe
Issue number4
Publication statusPublished - Apr 17 2013

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Virology


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