TY - JOUR
T1 - Identification of clonogenic common lymphoid progenitors in mouse bone marrow
AU - Kondo, Motonari
AU - Weissman, Irving L.
AU - Akashi, Koichi
N1 - Funding Information:
Correspondence should be addressed to K. A. (Akashi@Darwin.Stanford.edu). We thank S.-I. Nishikawa, N. Vita, and D. Cosman for anti-IL-7Rα antibody, recombinant human IL-7, and recombinant mouse SLF, respectively; L. Jerabek for capable laboratory management; L. Hidalgo and B. Lavarro for animal care; V. Braunstein for antibody preparation; S. Cheshier for cell cycle analysis; the staff at FACS facility for instruction of flow cytometer operation; and A. Schlageter for critically reviewing the manuscript. This work was mainly supported by NCI grant CA42551 to I. L. W. and in part by grant of Uehara Memorial Foundation to K. A.
PY - 1997/11/28
Y1 - 1997/11/28
N2 - The existence of a common lymphoid progenitor that can only give rise to T cells, B cells, and natural killer (NK) cells remains controversial and constitutes an important gap in the hematopoietic lineage maps. Here, we report fiat the Lin -IL-7R+Thy-1 -Sca-1(lo)c-Kit(lo) population from adult mouse bone marrow possessed a rapid lymphoid-restricted (T, B, and NK) reconstitution capacity in vivo but completely lacked myeloid differentiation potential either in vivo or in vitro. A single Lin-IL-7R+Thy-1-Sca- 1(lo)c-Kit(lo) cell could generate at least both T and B cells. These data provide direct evidence for the existence of common lymphoid progenitors in sites of early hematopoiesis.
AB - The existence of a common lymphoid progenitor that can only give rise to T cells, B cells, and natural killer (NK) cells remains controversial and constitutes an important gap in the hematopoietic lineage maps. Here, we report fiat the Lin -IL-7R+Thy-1 -Sca-1(lo)c-Kit(lo) population from adult mouse bone marrow possessed a rapid lymphoid-restricted (T, B, and NK) reconstitution capacity in vivo but completely lacked myeloid differentiation potential either in vivo or in vitro. A single Lin-IL-7R+Thy-1-Sca- 1(lo)c-Kit(lo) cell could generate at least both T and B cells. These data provide direct evidence for the existence of common lymphoid progenitors in sites of early hematopoiesis.
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U2 - 10.1016/S0092-8674(00)80453-5
DO - 10.1016/S0092-8674(00)80453-5
M3 - Article
C2 - 9393859
AN - SCOPUS:0030831130
SN - 0092-8674
VL - 91
SP - 661
EP - 672
JO - Cell
JF - Cell
IS - 5
ER -