Identification of cDNAs encoding the WD repeat protein and auxin-regulated protein in nicotiana glutinosa leaves induced in response to tobacco mosaic virus-infection at 25°C

Nicotiana glutinosa represents resistance to tobacco mosaic virus (TMV)- infection by causing a localized hypersensitive response (HR) at 25 °C. The fluorescent differential display (FDD) analysis followed by reverse transcription (RT)-PCR indicated that two transcripts encoding a WD-repeated protein and a putative auxin-regulated protein were significantly increased upon TMV-infection at 25 °C. The two fulllength cDNAs (Ng0881 and Ngl851), which encode the WD repeat protein and auxin-regulated protein, were cloned, and the complete nucleotide sequences were determined. The deduced amino acid sequence for Ng0881 has seven repetitive WD repeats, sharing 81% identical residues with the homologue from Oryza sativa, while that for Ng1851 shares 68% identical residues with the auxin-regulated protein from Oryza sativa. It could be speculated that translational products of the genes ng0881 and ng1851 may serve as signal mediators required for TMV-resistance.