Identification of cDNA sequences encoding the complement components of Zebrafish (Danio rerio)

Vo Kha Tam, Masakazu Tsujikura, Tomonori Somamoto, Miki Nakao

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Zebrafish can be utilized for immunological research as a fish model that allows gene targeting, but lacks enough molecular and sequence information on the complement system, a humoral innate immune factor. The present study aimed at identification of all the major complement components at the molecular level with phylogenetic evidence. Complementary DNA sequences encoding Clq, Clr/s, C2 (B/C2-B), C3, C4, C5, C6, C7, C8, C9, factor B (B/C2-A), the mannose-binding lectin (MBL), two MBL-associated serine proteases, properdin, and factor I were identified by cloning from the hepatopancreas and by database mining. As reported for carp and trout, the presence of two distinct isotypes were confirmed for C3, C4, and C7 also in zebrafish, indicating the ancient evolutionary origin of their diversifications in the vertebrate lineage.

Original languageEnglish
Pages (from-to)373-387
Number of pages15
JournalJournal of the Faculty of Agriculture, Kyushu University
Issue number2
Publication statusPublished - Oct 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science


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