Identification of Arabidopsis Genes Regulated by High Light-Stress Using cDNA Microarray

Mitsuhiro Kimura, Yoshiharu Y. Yamamoto, Motoaki Seki, Tetsuya Sakurai, Masakazu Sato, Tomoko Abe, Shigeo Yoshida, Katsushi Manabe, Kazuo Shinozaki, Minami Matsui

Research output: Contribution to journalArticlepeer-review

191 Citations (Scopus)

Abstract

In plants, excess light has the potential to damage the photosynthetic apparatus. The damage is caused in part by reactive oxygen species (ROS) generated by electrons leaking from the photosynthetic electron transport system. To investigate the mechanisms equipped in higher plants to reduce high light (HL) stress, we surveyed the response of 7000 Arabidopsis genes to HL, taking advantage of the recently developed microarray technology. Our analysis revealed that 110 genes had a positive response to a 3 h treatment at a light intensity of 150 W m-2. In addition to the scavenging enzymes of ROS, the genes involved in biosynthesis of lignins and flavonoids are activated by HL and actually resulted in increased accumulation of lignins and anthocyanins. Comparing the HL-responsive genes with drought-inducible genes identified with the same microarray system revealed a dense overlap between HL- and drought-inducible genes. In addition, we have identified 10 genes that showed upregulation y HL, drought, cold and also salt stress. These genes include RD29A, ERD7, ERD10, KIN1, LEA14 and COR15a, most of which are thought to be involved in the protection of cellular components.

Original languageEnglish
Pages (from-to)226-233
Number of pages8
JournalPhotochemistry and Photobiology
Volume77
Issue number2
DOIs
Publication statusPublished - Feb 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Radiation
  • Biochemistry
  • Physical and Theoretical Chemistry

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